Mangan Michael W, Byrne Gavin A, Meijer Wim G
Department of Industrial Microbiology, Conway Institute for Biomolecular and Biomedical Reseach, University College Dublin, Dublin 4, Ireland.
Antonie Van Leeuwenhoek. 2005 Feb;87(2):161-7. doi: 10.1007/s10482-004-3113-2.
Rhodococcus equi is an intracellular pathogen of macrophages, causing disease in young foals, humans, and sporadically other animals. Although R. equi is easy to grow and manipulate, the analysis of virulence is hampered by a lack of molecular tools. This paper describes the development of a number of versatile plasmids for use in R. equi. Plasmids pREV2 and pREV5 use origins of replication derived from the Mycobacterium fortuitum plasmids pAL5000 and pMF1. These plasmids and their derivatives are compatible in R. equi, allowing their use for analysis of gene function in trans. The stability of these plasmids in R. equi in the absence of selection for the plasmid borne antibiotic resistance markers, and their integrity following passage through Escherichia coli and R. equi was determined.
马红球菌是巨噬细胞的胞内病原体，可导致幼驹、人类发病，偶尔也会感染其他动物。尽管马红球菌易于培养和操作，但由于缺乏分子工具，其毒力分析受到阻碍。本文描述了一系列用于马红球菌的多功能质粒的构建。质粒pREV2和pREV5使用了源自偶然分枝杆菌质粒pAL5000和pMF1的复制起点。这些质粒及其衍生物在马红球菌中具有相容性，可用于反式分析基因功能。测定了这些质粒在不选择质粒携带的抗生素抗性标记的情况下在马红球菌中的稳定性，以及它们在通过大肠杆菌和马红球菌传代后的完整性。
