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RP4-oriT穿梭载体从大肠杆菌到产气荚膜梭菌的接合转移。

Conjugative transfer of RP4-oriT shuttle vectors from Escherichia coli to Clostridium perfringens.

作者信息

Lyras D, Rood J I

机构信息

Department of Microbiology, Monash University, Clayton, Victoria, Australia.

出版信息

Plasmid. 1998;39(2):160-4. doi: 10.1006/plas.1997.1325.

DOI:10.1006/plas.1997.1325
PMID:9514706
Abstract

A chromosomally integrated copy of the IncP plasmid RP4 was shown to mediate the conjugative transfer of shuttle plasmids containing an oriT site from Escherichia coli to the anaerobic pathogen Clostridium perfringens. Two versatile shuttle plasmids, pJIR1456 and pJIR1457, which will be invaluable for the introduction of cloned genes into C. perfringens, were constructed.

摘要

已证明 IncP 质粒 RP4 的染色体整合拷贝介导了含有 oriT 位点的穿梭质粒从大肠杆菌到厌氧病原体产气荚膜梭菌的接合转移。构建了两个通用穿梭质粒 pJIR1456 和 pJIR1457，它们对于将克隆基因导入产气荚膜梭菌将具有极高价值。

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