Giri A K, Sivam S S, Khan K A, Sethi N
Division of Toxicology, Central Drug Research Institute, Lucknow, India.
Environ Mol Mutagen. 1992;19(3):223-6. doi: 10.1002/em.2850190306.
Sister chromatic exchanges (SCE) and chromosome aberrations (CA) in mice after in vivo exposure of Green S were carried out following single acute treatment. Except for the lowest dose (25 mg/kg body weight) a significant increase in the SCEs were observed in all the other doses (50, 100, and 200 mg/kg) tested. In CA study two higher doses (200 and 400 mg/kg) showed a significant increase in CA when compared with control. The minimum effective dose which induced SCE and CA was 50 and 200 mg/kg of body weight, respectively. The trend tests for the evidence of dose response effects were also significant for both SCE and CA. No significant differences were observed in cell replication kinetic (RI) analysis. A significant increase in the mitotic index (MI) was also observed in the highest dose (400 mg/kg) tested when compared with control. Thus the present study indicates that Green S can induce both SCE and CA in vivo in bone marrow cells of mice.
在对小鼠进行单次急性处理后，对其体内暴露于绿色素S后的姐妹染色单体交换（SCE）和染色体畸变（CA）情况进行了研究。除最低剂量（25毫克/千克体重）外，在所测试的所有其他剂量（50、100和200毫克/千克）下均观察到SCE显著增加。在染色体畸变研究中，与对照组相比，两个较高剂量（200和400毫克/千克）显示出染色体畸变显著增加。诱导SCE和CA的最低有效剂量分别为50毫克/千克和200毫克/千克体重。SCE和CA的剂量反应效应证据的趋势检验也均具有显著性。在细胞复制动力学（RI）分析中未观察到显著差异。与对照组相比，在测试的最高剂量（400毫克/千克）下也观察到有丝分裂指数（MI）显著增加。因此，本研究表明绿色素S可在小鼠骨髓细胞体内诱导SCE和CA。
