Aida M, Irié T, Aida T, Tachikawa T
Department of Oral Pathology, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
J Dent Res. 2005 Mar;84(3):234-9. doi: 10.1177/154405910508400305.
Protein kinase C (PKC) is an important molecule involved in various cell function, and mediates induced secretion of vascular endothelial growth factor (VEGF). It is hypothesized that PKC and VEGF may be associated with tooth development. Using the laser microdissection method and real-time reverse-transcription-polymerase chain-reaction (RT-PCR), we investigated the expression of PKC betaI and betaII, VEGF, and amelogenin (used as a marker of differentiation to ameloblasts) in the inner and outer enamel epithelia, stellate reticulum, and dental papilla in each stage of the dental germ. We found that the expression levels of PKC betaI and betaII were increased in the inner enamel epithelium during the early bell stage. In addition, the increased expression levels of PKC betaI and betaII were accompanied by increased VEGF expression. These results indicate that PKC betaI, betaII, and VEGF are closely associated with the differentiation of the inner enamel epithelium to ameloblasts.
蛋白激酶C（PKC）是参与多种细胞功能的重要分子，介导血管内皮生长因子（VEGF）的诱导分泌。据推测，PKC和VEGF可能与牙齿发育有关。我们采用激光显微切割法和实时逆转录-聚合酶链反应（RT-PCR），研究了牙胚各阶段内釉上皮、外釉上皮、星网状层和牙乳头中PKC βI和βII、VEGF以及釉原蛋白（用作成釉细胞分化标志物）的表达情况。我们发现，在钟状早期阶段，内釉上皮中PKC βI和βII的表达水平升高。此外，PKC βI和βII表达水平的升高伴随着VEGF表达的增加。这些结果表明，PKC βI、βII和VEGF与内釉上皮向成釉细胞的分化密切相关。
