Guillam M T, Beuron F, Grandin N, Hubert J F, Boisseau C, Cavalier A, Couturier A, Gouranton J, Thomas D
Laboratoire de Biologie Cellulaire URA CNRS No. 256, Université de Rennes 1, France.
Exp Cell Res. 1992 Jun;200(2):301-5. doi: 10.1016/0014-4827(92)90176-9.
The highly specialized membranes of the filter chamber found in the digestive tract of some homopteran insects could represent a favorable material for characterizing water channels. In order to demonstrate that membrane proteins of this epithelial complex serve as water channels, we have investigated the membrane permeability for water in Xenopus oocytes injected with RNA isolated from the filter chamber. Volumes of oocytes injected with filter chamber RNA were increased by 15% following a 16-min osmotic shock, while volumes of oocytes injected with RNA from midgut not of filter chamber or with water were increased only by 8.5 and 10%, respectively. This significant difference in oocyte swelling leads us to conclude that RNA isolated from the filter chamber contains mRNA coding for water channel proteins.
在一些同翅目昆虫消化道中发现的滤室高度特化的膜，可能是用于表征水通道的良好材料。为了证明这种上皮复合体的膜蛋白充当水通道，我们研究了注射从滤室分离的RNA的非洲爪蟾卵母细胞对水的膜通透性。在16分钟的渗透压休克后，注射滤室RNA的卵母细胞体积增加了15%，而注射非滤室中肠RNA或水的卵母细胞体积仅分别增加了8.5%和10%。卵母细胞肿胀的这一显著差异使我们得出结论，从滤室分离的RNA包含编码水通道蛋白的mRNA。
