Furia M, D'Avino P P, Digilio F A, Crispi S, Giordano E, Polito L C
Dipartimento di Genetica, Biologia Generale e Molecolare, Università di Napoli, Italia.
Genet Res. 1992 Feb;59(1):19-26. doi: 10.1017/s0016672300030135.
The Drosophila melanogaster ecd1 mutation causes a severe temperature-sensitive deficiency in the titre of the steroid hormone ecdysone. This mutation was used to investigate the role of ecdysone in both the transcription of the genes mapped at the 3C11-12 intermoult puff region and the puff formation. Thoroughly synchronized ecd1 larvae were shifted to the non-permissive temperature at various times of the development; after 24 or 48 h, the levels of the transcripts derived from Sgs-4, Pig-1 and ng-1, the three genes located at the 3C11-12 polytene bands, were determined. The results showed that the levels of the transcripts encoded by Pig-1 and ng-1 are unaffected by the drop in the ecdysone titre occurring in non-permissive conditions whereas the amount of Sgs-4 mRNA is greatly reduced. These data clearly indicate that transcription of the three genes mapped within the puff region is affected differently by the hormone. Furthermore, ecd1 larvae cultured at the non-permissive temperature show a prominent puff at the 3C11-12 polytene bands, indicating that ecdysone is not essential for puff induction and that puff size is not simply correlated with high-level Sgs-4 transcription.
果蝇黑腹ecd1突变导致类固醇激素蜕皮激素滴度出现严重的温度敏感缺陷。该突变用于研究蜕皮激素在位于3C11 - 12龄间蜕皮疏松区基因转录以及疏松形成中的作用。将完全同步化的ecd1幼虫在发育的不同时间转移到非允许温度；24或48小时后，测定位于3C11 - 12多线带的三个基因Sgs - 4、Pig - 1和ng - 1的转录本水平。结果表明，在非允许条件下发生的蜕皮激素滴度下降对Pig - 1和ng - 1编码的转录本水平没有影响，而Sgs - 4 mRNA的量则大大减少。这些数据清楚地表明，位于疏松区内的三个基因的转录受激素的影响不同。此外，在非允许温度下培养的ecd1幼虫在3C11 - 12多线带处显示出明显的疏松，这表明蜕皮激素对于疏松诱导不是必需的，并且疏松大小与高水平的Sgs - 4转录并非简单相关。
