Belyaeva E S, Vlassova I E, Biyasheva Z M, Kakpakov V T, Richards G, Zhimulev I F
Chromosoma. 1981;84(2):207-19. doi: 10.1007/BF00399132.
Larvae homozygous or hemizygous for the l(1)t435 mutation located within the early ecdysteroid puff 2B5, or carrying a deletion of the 2B5 band, die at the end of the third larval instar. In the salivary gland chromosomes of these larvae only intermoult puffs are detected. If these salivary glands are incubated in vitro with 20-OH ecdysone for 6 h the intermoult puff 68 C remains large, some early puffs (74EF and 75B) are induced to 30-40% of their normal size, other early (63F) and all late puffs (62E, 78D, 82F and 63E) are not induced at all. Puff 2B5 reaches its normal size but does not regress after 6 h incubation with 20-OH ecdysone, as it does in normal stocks. The data obtained in this study show the existence of a locus (or loci) in the band (puff) 2B5 which is necessary for the normal response of the salivary gland chromosomes to the hormone 20-OH ecdysone.
对于位于早期蜕皮甾类胀泡2B5内的l(1)t435突变纯合或半合子的幼虫，或者携带2B5带缺失的幼虫，在第三龄幼虫末期死亡。在这些幼虫的唾液腺染色体中，仅检测到蜕皮间期胀泡。如果将这些唾液腺在体外与20-羟基蜕皮酮一起孵育6小时，蜕皮间期胀泡68C仍然很大，一些早期胀泡（74EF和75B）被诱导至正常大小的30-40%，其他早期胀泡（63F）和所有晚期胀泡（62E、78D、82F和63E）根本未被诱导。胀泡2B5达到其正常大小，但在与20-羟基蜕皮酮孵育6小时后不像在正常品系中那样消退。本研究获得的数据表明，在带（胀泡）2B5中存在一个位点（或多个位点），这对于唾液腺染色体对激素20-羟基蜕皮酮的正常反应是必需的。
