Cavagión Laura, Perez Alicia, Santillan Graciela, Zanini Fabián, Jensen Oscar, Saldía Luisa, Diaz Mario, Cantoni Gustavo, Herrero Eduardo, Costa María T, Volpe Marcela, Araya Daniel, Rubianes Nicolás Alvarez, Aguado Carlos, Meglia Gustavo, Guarnera Eduardo, Larrieu Edmundo
School of Veterinary Sciences, National University of La Pampa, Argentina.
Vet Parasitol. 2005 Mar 10;128(1-2):73-81. doi: 10.1016/j.vetpar.2004.11.006. Epub 2004 Dec 30.
In 2000 Guarnera et al. proposed using ELISA in canine faeces collected from the ground to detect dogs infected with Echinococcus granulosus, thus determining sheep farms with active transmission. The objective was to evaluate the prevalence of E. granulosus infection in sheep farms of the Patagonia. Sheep farms were randomly selected in the Provinces of Río Negro, Chubut, Neuquén, Santa Cruz and Tierra del Fuego (areas with control programs) and La Pampa (comparison area). From one to three samples of fecal matter were obtained for each sheep farm, which were processed by means of copro-ELISA test with confirmation of positive samples by copro-Western blot. A total of 1042 samples were obtained from 352 sheep farms, 26 (7.3%) proving positive. Of these 5 (6.3%) were from La Pampa, 9 (13.8%) from Neuquén, 4 (4.7%) from Río Negro, 2 (2.9%) from Chubut, 1 (5.9%) from Santa Cruz and 5 (13.9%) from Tierra del Fuego. The identification of parasitized dogs is an essential activity upon which rests the strategy of control and surveillance. Arecoline tests or coproantigen test with fecal matter obtained directly from the dog contribute information on individual prevalence, while the use of coproantigens detected in ground-collected samples transfers the dog unit of observation to units of greater epidemiological value. In the present experience, the technique employed seems promising for its application in systems of epidemiological surveillance of cystic echinococcosis and in drawing a baseline on which to measure the progress of control programs in the Argentine Patagonia in subsequent years.
2000年，瓜尔内拉等人提议使用酶联免疫吸附测定法（ELISA）检测从地面采集的犬粪便中的细粒棘球绦虫感染犬，从而确定存在活跃传播的养羊场。目的是评估巴塔哥尼亚地区养羊场中细粒棘球绦虫感染的流行情况。在里奥内格罗省、丘布特省、内乌肯省、圣克鲁斯省和火地岛省（设有控制项目的地区）以及潘帕斯省（对照地区）随机选择养羊场。每个养羊场采集1至3份粪便样本，通过粪便酶联免疫吸附测定试验进行处理，并通过粪便免疫印迹法确认阳性样本。共从352个养羊场采集了1042份样本，其中26份（7.3%）检测呈阳性。其中，5份（6.3%）来自潘帕斯省，9份（13.8%）来自内乌肯省，4份（4.7%）来自里奥内格罗省，2份（2.9%）来自丘布特省，1份（5.9%）来自圣克鲁斯省，5份（13.9%）来自火地岛省。识别受寄生虫感染的犬是控制和监测策略的一项基本活动。对直接从犬获取的粪便进行槟榔碱试验或粪便抗原试验可提供个体感染率信息，而对从地面采集样本中检测到的粪便抗原的使用则将观察犬的单位转变为具有更大流行病学价值的单位。在本次试验中，所采用的技术似乎有望应用于囊型包虫病的流行病学监测系统，并为衡量阿根廷巴塔哥尼亚地区后续年份控制项目的进展情况提供一个基线。
