Soriano S V, Pierangeli N B, Pianciola L, Mazzeo M, Lazzarini L E, Saiz M S, Kossman A V, Bergagna H F J, Chartier K, Basualdo J A
Cátedra de Microbiología y Parasitología, Escuela de Medicina, Universidad Nacional del Comahue. Buenos Aires 1400, (8300) Neuquén, Argentina.
Parasitol Int. 2010 Dec;59(4):626-8. doi: 10.1016/j.parint.2010.07.003. Epub 2010 Jul 25.
Human cystic echinococcosis is a highly endemic zoonotic disease in the province of Neuquén, Patagonia Argentina, although a hydatid control programme has been carried out since 1970. Human infection due to Echinococcus canadensis (G6 genotype) is frequent in Neuquén. However, the reservoir for this species remains undetermined in a region where camels are absent. We investigated the fertility, viability and molecular epidemiology of hydatid cysts obtained from local goats, pigs and sheep in order to identify the possible reservoirs of E. canadensis (G6). We also analyzed isolates from infected dogs. A total of 67 isolates were identified by the DNA sequencing of the mitochondrial cytochrome c oxidase subunit 1 gene. Cysts from sheep (n=16), goats (n=23) and pigs (n=18) and adult worms from 10 infected dogs were analyzed. The fertility of the hydatid cysts was 78.6%; 90.4% and 94.4% for sheep, goats and pigs, respectively. We detected E. canadensis (G6) in 21 of 23 goat samples and in 1 dog isolate, E. canadensis (G7) in all the pig isolates, E. granulosus sensu stricto (G3) in 1 sheep and the G1 genotype in 15 sheep, 2 goats and 9 dog samples. The G1 haplotypes included the common sheep strain sequence and 2 microvariants of this sequence. E. granulosus sensu stricto (G3) is described for the first time in South America. We conclude that goats act as reservoir for E. canadensis (G6) in Neuquén, and that control strategies may have to be adapted to local molecular epidemiology to improve the control of parasite transmission.
人体囊型包虫病在阿根廷巴塔哥尼亚内乌肯省是一种高度流行的人畜共患病，尽管自1970年以来已开展了一项包虫控制计划。在阿根廷内乌肯省，由加拿大棘球绦虫（G6基因型）引起的人体感染很常见。然而，在没有骆驼的地区，该物种的储存宿主仍未确定。我们调查了从当地山羊、猪和绵羊获得的包虫囊肿的繁殖力、活力和分子流行病学，以确定加拿大棘球绦虫（G6）可能的储存宿主。我们还分析了来自受感染犬的分离株。通过线粒体细胞色素c氧化酶亚基1基因的DNA测序共鉴定出67个分离株。分析了来自绵羊（n = 16）、山羊（n = 23）和猪（n = 18）的囊肿以及10只受感染犬的成虫。包虫囊肿的繁殖力分别为：绵羊78.6%、山羊90.4%和猪94.4%。我们在23份山羊样本中的21份以及1份犬分离株中检测到加拿大棘球绦虫（G6），在所有猪分离株中检测到加拿大棘球绦虫（G7），在1只绵羊中检测到狭义细粒棘球绦虫（G3），在15只绵羊、2只山羊和9份犬样本中检测到G1基因型。G1单倍型包括常见的绵羊株序列及其2个微变体。狭义细粒棘球绦虫（G3）在南美洲首次被描述。我们得出结论，在阿根廷内乌肯省，山羊是加拿大棘球绦虫（G6）的储存宿主，并且控制策略可能必须根据当地分子流行病学进行调整，以改善对寄生虫传播的控制。
