Vanek Tomás, Halík Jan, Vanková Radmila, Valterová Irena
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic.
Biosci Biotechnol Biochem. 2005 Feb;69(2):321-5. doi: 10.1271/bbb.69.321.
Both enantiomers and the racemate of alpha-pinene were transformed by Picea abies cells immobilised on alginate. The main products were cis- and trans-verbenol, the later being further transformed to verbenone. The enantiomeric purity of each product more or less corresponded to that of the substrate. Transformation by free cells was faster than that by the immobilised cells. The ratio of products differed to some extent between the transformation by free and immobilised cells.
α-蒎烯的对映体和外消旋体都被固定在藻酸盐上的欧洲云杉细胞转化。主要产物是顺式和反式马鞭草烯醇，后者进一步转化为马鞭草烯酮。每种产物的对映体纯度或多或少与底物的对映体纯度相对应。游离细胞的转化速度比固定化细胞的转化速度快。游离细胞和固定化细胞转化之间的产物比例在一定程度上有所不同。
