Department of Biochemistry, Instituto de Química-UFRJ, CT, Rio de Janeiro, Brazil.
Appl Biochem Biotechnol. 2011 Jun;164(4):514-23. doi: 10.1007/s12010-010-9153-3. Epub 2011 Jan 15.
The main objective of this work is to present the optimization of the biotransformation of R-(+)-limonene and (-)-β-pinene aiming at the production of α-terpineol by strains of fungal and yeasts previously isolated by our research group using the methodology of experimental design. New optimized experimental data on α-terpineol production by the biotransformation of R-(+)-limonene and (-)-β-pinene using newly isolated microorganisms are reported in this work. Conversion of about 1,700 mg/L was achieved when R-(+)-limonene was used as substrate and the newly isolated strain 05.01.35 as microorganism at the central point of the experimental design, corresponding to a substrate concentration of 1.75%, mass of inoculum of 2 g, and substrate to ethanol volume ratio of 1:1. The same experimental condition led to higher conversions when (-)-β-pinene was used as substrates and the strains coded as 04.05.08 and 01.04.03 as microorganism. Here, conversions of about 770 mg/L were achieved.
本工作的主要目的是展示通过实验设计方法，对先前由我们的研究小组分离的真菌和酵母菌株进行 R-(+)-柠檬烯和（-）-β-蒎烯的生物转化，以生产α-松油醇的优化。本文报道了使用新分离的微生物对 R-(+)-柠檬烯和（-）-β-蒎烯进行生物转化生产α-松油醇的新优化实验数据。当使用 R-(+)-柠檬烯作为底物，并且在实验设计的中心点使用新分离的菌株 05.01.35 作为微生物时，实现了约 1700mg/L 的转化率，对应于底物浓度为 1.75%，接种量为 2g，以及底物与乙醇体积比为 1:1。在相同的实验条件下，当使用（-）-β-蒎烯作为底物，并且使用编码为 04.05.08 和 01.04.03 的菌株作为微生物时，转化率更高。在这里，实现了约 770mg/L 的转化率。
