Jagadeeswaran Pudur
Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA.
Curr Opin Hematol. 2005 Mar;12(2):149-52. doi: 10.1097/01.moh.0000155122.27551.6a.
In the past eight years our laboratory has developed the zebrafish model to study hemostasis and thrombosis. The purpose of this review is to explore current developments involving the zebrafish model in the study of hemostasis and thrombosis because the time is now ripe to apply this model to identify novel players that participate in hemostasis and thrombosis.
In the past twelve months, three papers appeared in the hemostasis and thrombosis area using the zebrafish model. The first one is a review article that summarizes establishment of the zebrafish model to study hemostasis and thrombosis. The second study is a methodological paper describing assays for measuring hemostasis and thrombosis by inducing vascular occlusion in zebrafish larvae. The third paper describes a knockdown of prothrombin in zebrafish, which recapitulates knockout studies in mouse, and marks the beginning of studies in the hemostasis and thrombosis area by this new knockdown technology. In addition to the above papers, there is one abstract that describes kinetics of thrombocyte and thrombocyte-microparticle recruitment in laser-induced arterial thrombus formation in zebrafish.
With the above advances, the zebrafish model has now matured to the point that it can address more important questions in the hemostasis and thrombosis area using genetic approaches. This review therefore summarizes the issues described in the above papers along with thoughts about future progress of the zebrafish model as a tool to study hemostasis and thrombosis.
在过去八年里，我们实验室开发了斑马鱼模型来研究止血和血栓形成。这篇综述的目的是探讨斑马鱼模型在止血和血栓形成研究中的当前进展，因为现在将该模型应用于识别参与止血和血栓形成的新因子的时机已经成熟。
在过去十二个月里，有三篇使用斑马鱼模型的论文出现在止血和血栓形成领域。第一篇是一篇综述文章，总结了用于研究止血和血栓形成的斑马鱼模型的建立。第二项研究是一篇方法学论文，描述了通过诱导斑马鱼幼虫血管闭塞来测量止血和血栓形成的实验方法。第三篇论文描述了斑马鱼中凝血酶原的敲低，这重现了小鼠中的基因敲除研究，并标志着这种新的敲低技术在止血和血栓形成领域研究的开端。除了上述论文外，还有一篇摘要描述了斑马鱼激光诱导动脉血栓形成过程中血小板和血小板微粒募集的动力学。
随着上述进展，斑马鱼模型现已成熟，能够使用遗传学方法解决止血和血栓形成领域中更重要的问题。因此，本综述总结了上述论文中描述的问题以及关于斑马鱼模型作为研究止血和血栓形成工具的未来进展的思考。
