Jagadeeswaran Pudur, Carrillo Maira, Radhakrishnan Uvaraj P, Rajpurohit Surendra K, Kim Seongcheol
Department of Biological Sciences, University of North Texas, Denton, Texas, USA.
Methods Cell Biol. 2011;101:197-203. doi: 10.1016/B978-0-12-387036-0.00009-8.
In the event of injury to the vasculature in vertebrate organisms bleeding is stopped by a defense mechanism called hemostasis. Even though biochemical studies characterized a number of factors, classical genetic methods have not been applied to study hemostasis. We introduced zebrafish as an animal model to study genetics of hemostasis. To conduct genetic studies of hemostasis, we required a global screening method to address all the factors of hemostasis such as those present in plasma, in platelets or those present in the endothelium. Therefore, we developed a global laser induced thrombosis method which can assay all these components. In this paper, we describe the principle of this method as well as provide the detailed protocol so this could be used as a screening tool to measure hemostasis in any laboratory.
在脊椎动物机体中，一旦脉管系统受伤，一种名为止血的防御机制会阻止出血。尽管生化研究已鉴定出许多因子，但经典遗传学方法尚未应用于止血研究。我们引入斑马鱼作为研究止血遗传学的动物模型。为了进行止血的遗传学研究，我们需要一种全面的筛选方法来研究止血的所有因子，比如血浆中、血小板中或内皮细胞中存在的因子。因此，我们开发了一种全面的激光诱导血栓形成方法，该方法可以检测所有这些成分。在本文中，我们描述了此方法的原理，并提供详细的实验方案，以便其可作为一种筛选工具，供任何实验室用于测量止血情况。
