Tesseromatis C, Kotsiou A, Mourouzis C, Saranteas Th, Potamianou A, Vairactaris E
Department of pharmacology, School of Medicine, University of Athens, Greece.
Eur J Drug Metab Pharmacokinet. 2004 Oct-Dec;29(4):231-3. doi: 10.1007/BF03190604.
Circadian rhythm may induce alterations of the pharmacokinetic properties of several drugs in clinical use. The aim of the study was to investigate whether lighting conditions alter the quinolone (pefloxacin) levels in serum and tissues and to determine any accumulation of the drug in the skin. Thirty male Wistar rats were divided into groups A, B, C, (n=10). The animals of group A were housed under 12h light/12h dark conditions, group B under 24h UV and group C was kept in complete darkness. All animals received 5 doses of 11mg/Kg pefloxacin every 8h for 48h.Pefloxacin levels were determined in serum, skin and femur by the inhibition zone in E.coli. in vitro. Pefloxacin concentrations in serum were increased in 24h darkness living status and decreased in 24h UV conditions as compared to group A animals. Additionally, both skin and femur pefloxacin levels were decreased under dark and UV conditions. In conclusion total light as well as total dark exposure may lead to pefloxacin pharmacokinetic changes which may have implications in the effectiveness of the drug in tissues.
昼夜节律可能会导致几种临床常用药物的药代动力学特性发生改变。本研究的目的是调查光照条件是否会改变血清和组织中的喹诺酮类药物（培氟沙星）水平，并确定该药物在皮肤中的蓄积情况。将30只雄性Wistar大鼠分为A、B、C组（每组n = 10）。A组动物饲养在12小时光照/12小时黑暗条件下，B组饲养在24小时紫外线照射条件下，C组饲养在完全黑暗环境中。所有动物每8小时接受5剂11mg/Kg的培氟沙星，持续48小时。通过体外大肠杆菌抑菌圈法测定血清、皮肤和股骨中的培氟沙星水平。与A组动物相比，在24小时黑暗环境下生活的动物血清中培氟沙星浓度升高，在24小时紫外线照射条件下血清中培氟沙星浓度降低。此外，在黑暗和紫外线条件下，皮肤和股骨中的培氟沙星水平均降低。总之，全光照以及全黑暗暴露可能会导致培氟沙星药代动力学变化，这可能会影响该药物在组织中的有效性。
