Hubert Casey, Shen Yin, Voordouw Gerrit
Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4.
Biodegradation. 2005 Feb;16(1):11-22. doi: 10.1007/s10531-003-0471-4.
The effects of trichloroethylene (TCE) on microbial community composition were analyzed by reverse sample genome probing. Soil enrichments were incubated in dessicators containing an organic phase of either 1 or 10% (w/w) toluene in vacuum pump oil, delivering constant equilibrium aqueous concentrations of 16 and 143 mg/l, respectively. Increasing the equilibrium aqueous concentration of TCE from 0 to 10 mg/l led to shifts in community composition at 16, but not at 143 mg/l of toluene. In closed system co-degradation studies, TCE at an aqueous concentration of 1 mg/l was effectively degraded by toluene-degrading soil enrichments once the aqueous toluene concentration dropped below 25 mg/l. Little TCE degradation was observed at higher toluene concentrations (50-250 mg/l). The results indicate that TCE changes microbial community composition under conditions where it is being actively metabolized.
采用反向样品基因组探测法分析了三氯乙烯（TCE）对微生物群落组成的影响。土壤富集培养物在装有1%或10%（w/w）甲苯的真空泵油有机相的干燥器中进行培养，分别提供16 mg/l和143 mg/l的恒定平衡水相浓度。将TCE的平衡水相浓度从0提高到10 mg/l，导致在甲苯浓度为16 mg/l时群落组成发生变化，但在143 mg/l时未发生变化。在封闭系统共降解研究中，一旦水相甲苯浓度降至25 mg/l以下，甲苯降解土壤富集培养物就能有效降解水相浓度为1 mg/l的TCE。在较高甲苯浓度（50 - 250 mg/l）下，未观察到TCE的明显降解。结果表明，TCE在其被积极代谢的条件下会改变微生物群落组成。
