Ahn Yeonghee, Jung Haeryong, Tatavarty Rameshwar, Choi Heechul, Yang Ji-Won, Kim In S
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon 305-701, Korea.
Biodegradation. 2005 Feb;16(1):45-56. doi: 10.1007/s10531-004-0428-2.
This study was performed to investigate the petroleum hydrocarbon (PH) degradative potential of indigenous microorganisms in ozonated soil to better develop combined pre-ozonation/bioremediation technology. Diesel-contaminated soils were ozonated for 0-900 min. PH and microbial concentrations in the soils decreased with increased ozonation time. The greatest reduction of total PH (TPH, 47.6%) and aromatics (11.3%) was observed in 900-min ozonated soil. The number of total viable heterotrophic bacteria decreased by three orders of magnitude in the soil. Ozonated soils were incubated for 9 weeks for bioremediation. The number of microorganisms in the soils increased during the incubation period, as monitored by culture- and nonculture-based methods. The soils showed additional PH-removal during incubation, supporting the presence of PH-degraders in the soils. The highest removal (25.4%) of TPH was observed during the incubation of 180-min ozonated soil during the incubation while a negligible removal was shown in 900-min ozonated soil. This negligible removal could be explained by the existence of relatively few or undetected PH-degraders in 900-min ozonated soil. After a 9-week incubation of the ozonated soils, 180-min ozonated soil showed the lowest TPH concentration, suggesting that appropriate ozonation and indigenous microorganisms survived ozonation could enhance remediation of PH-contaminated soil. Microbial community composition in 9-week incubated soils revealed a slight difference between 900-min ozonated and unozonated soils, as analyzed by whole cell hybridization. Taken together, this study provided insight into indigenous microbial potential to degrade PH in ozonated soils.
本研究旨在调查臭氧化土壤中本地微生物对石油烃（PH）的降解潜力，以更好地开发臭氧预氧化/生物修复联合技术。对受柴油污染的土壤进行0至900分钟的臭氧化处理。土壤中PH和微生物浓度随臭氧化时间的增加而降低。在臭氧化900分钟的土壤中，总PH（TPH，降低了47.6%）和芳烃（降低了11.3%）的降幅最大。土壤中总活异养细菌数量减少了三个数量级。对臭氧化土壤进行9周的生物修复培养。通过基于培养和非培养的方法监测，培养期间土壤中的微生物数量增加。土壤在培养期间显示出额外的PH去除，这支持了土壤中存在PH降解菌。在培养期间，180分钟臭氧化土壤培养时TPH的去除率最高（25.4%），而900分钟臭氧化土壤的去除率可忽略不计。这种可忽略不计的去除可以用900分钟臭氧化土壤中相对较少或未检测到的PH降解菌来解释。臭氧化土壤经过9周培养后，180分钟臭氧化土壤的TPH浓度最低，这表明适当的臭氧化处理以及在臭氧化过程中存活下来的本地微生物可以增强对受PH污染土壤的修复。通过全细胞杂交分析，9周培养土壤中的微生物群落组成显示，900分钟臭氧化土壤和未臭氧化土壤之间存在细微差异。综上所述，本研究深入了解了臭氧化土壤中本地微生物降解PH的潜力。
