Jung Haeryong, Ahn Yeonghee, Choi Heechul, Kim In S
Department of Civil Engineering, University of Waterloo, Waterloo, Ont., Canada N2L 3G1.
Chemosphere. 2005 Nov;61(7):923-32. doi: 10.1016/j.chemosphere.2005.03.038. Epub 2005 Apr 26.
Soil column experiments were conducted to investigate the effects of chemical oxidation on the survival of indigenous microbes (i.e., heterotrophic microbes, phenanthrene-degrading microbes, and alkane-degrading microbes) for field soil contaminated with diesel fuel. Rapid decreases of total petroleum hydrocarbons (TPH) and aromatics of diesel fuel were observed within the first 60 min of ozone injection; after 60 min, TPH and aromatics decreased asymptotically with ozonation time. The three types of indigenous microbes treated were very sensitive to ozone in the soil column experiment, hence the microbial population decreased exponentially with ozonation time. The numbers of heterotrophic, alkane-degrading, and phenanthrene-degrading bacteria were reduced from 10(8) to 10(4), 10(7) to 10(3), and 10(6) CFU g soil(-1) to below detection limit after 900 min of ozonation, respectively. Except for the soil sample ozonated for 900 min, incubation of ozone-treated soil samples that were not limited by oxygen diffusion showed further removal of TPH. The soil samples that were ozonated for 180 min exhibited the lowest concentration of TPH and the highest regrowth rate of the heterotrophic and alkane-degrading populations after the 9 weeks of incubation.
进行了土柱实验，以研究化学氧化对受柴油污染的田间土壤中本地微生物（即异养微生物、菲降解微生物和烷烃降解微生物）存活的影响。在注入臭氧的前60分钟内，观察到柴油中总石油烃（TPH）和芳烃迅速减少；60分钟后，TPH和芳烃随臭氧化时间呈渐近减少。在土柱实验中，所处理的三种本地微生物对臭氧非常敏感，因此微生物数量随臭氧化时间呈指数下降。经过900分钟臭氧化后，异养细菌、烷烃降解细菌和菲降解细菌的数量分别从10⁸降至10⁴、从10⁷降至10³以及从10⁶CFU g土壤⁻¹降至检测限以下。除了经过900分钟臭氧化的土壤样品外，对不受氧扩散限制的臭氧处理土壤样品进行培养后，TPH进一步减少。经过180分钟臭氧化的土壤样品在培养9周后，TPH浓度最低，异养和烷烃降解菌群的再生长速率最高。
