Zhuang Jiaju, Wang Zhiling, Liu Jingdong, Gou Lingyan
Bethune Medical College, Shijiazhuang 050081, China.
Wei Sheng Yan Jiu. 2004 Nov;33(6):687-9.
To investigate the effects of superoxide dismutase (SOD) and reducing iron load on experimental immunological liver injury in rat.
48 male Wistar rats were divided into six groups randomly. The animal model of iron low-load was created by intravasation of deferoxamine (DFO) or phlebotomy respectively. Immunological liver damage model was reproduced by injection of lipopolysaccharide (LPS) and BCG (Bacilli Calmette Guerin). The following parameters were determined such as serum iron (SI), transferrin (TRF) concentration, total proteins (TP) volume, the serum activities of aspartate aminotransferase (AST), and malondialdehyde (MDA) content, the activities of superoxide dismutase and iron deposition (HIC) in liver tissue extracts.
In comparison with blank control group, the SI levels lowered (P < 0.05) in pure DFO group, pure phlebotomy group and liver injury group. Compared with each own control groups, the serum activities of AST in all injury groups augmented and the levels of TP in them reduced significantly. The increased magnitude of the activities of AST and the decreased magnitude of TP levels in DFO injury group and phlebotomy injury group were less than those of pure injury group (P < 0.05). In comparison with the control group, the MDA levels in pure DFO and phlebotomy groups declined significantly and the activities of SOD in them had no significant difference. Compared with each own control groups, the MDA levels augmented and the activities of SOD reduced significantly in all injury groups.
SOD activity reduces in immunological liver injury in rat. Iron low-load can mitigate the severity of liver injury.
探讨超氧化物歧化酶（SOD）及降低铁负荷对大鼠实验性免疫性肝损伤的影响。
将48只雄性Wistar大鼠随机分为6组。分别通过静脉注射去铁胺（DFO）或放血建立低铁负荷动物模型。通过注射脂多糖（LPS）和卡介苗（BCG）复制免疫性肝损伤模型。测定以下参数，如血清铁（SI）、转铁蛋白（TRF）浓度、总蛋白（TP）含量、天冬氨酸转氨酶（AST）血清活性、丙二醛（MDA）含量、超氧化物歧化酶活性以及肝组织提取物中的铁沉积（HIC）。
与空白对照组相比，单纯DFO组、单纯放血组和肝损伤组的SI水平降低（P<0.05）。与各自的对照组相比，所有损伤组的AST血清活性升高，TP水平显著降低。DFO损伤组和放血损伤组AST活性升高幅度和TP水平降低幅度均小于单纯损伤组（P<0.05）。与对照组相比，单纯DFO组和放血组的MDA水平显著下降，其SOD活性无显著差异。与各自的对照组相比，所有损伤组的MDA水平升高，SOD活性显著降低。
大鼠免疫性肝损伤时SOD活性降低。低铁负荷可减轻肝损伤的严重程度。
