[建立连续繁殖第三代的SD大鼠长期硒缺乏动物模型]

[Established prolonged selenium deficiency of the SD rat animal model breed third generation in series].

作者信息

Su Min, Zhang Ruijuan, Tian Dongping, Guo Xiong, Yu Yan, Wang Yan

机构信息

Department of Pathology, Medical College of Shantou University, Shantou 515031, China.

出版信息

Wei Sheng Yan Jiu. 2004 Nov;33(6):705-10.

PMID:15727183

Abstract

OBJECTIVE

To establish selenium deficiency of the breed third generation SD rats animal model in series.

METHODS

A Se-deficient diet mainly based on Se-deficient yeast. Groups of six female and six male SD rats original generation (F0) were fed on one of the five diets: (1) selenium- and iodine-adequate control group (Se+I+ group. Selenium: 0.1-0.3 microg/g, iodine > or = 0.2 microg/g), (2) selenium-deficient iodine-adequate group 1 (Se-I+ group. Selenium: 0.02 microg/g, iodine > or = 0.2 microg/g), (3) selenium-deficient iodine-adequate group 2 (Se-I+ group2. Selenium: 0.005 microg/g, iodine > or = 0.2 microg/g), (4) selenium-adequate iodine-deficient group (Se+I- group. Selenium: 0.1-0.3 microg/g, iodine: 0.04 microg/g), (5) selenium- and iodine-deficient group (Se-I- group. Selenium: 0.01 microg/g, iodine: 0.04 microg/g). Selenium-deficient iodine-adequate group2 had been deled since generation 1 (F1). Serum Se and thyroid hormone concentrations, whole blood glutathione peroxidase (GSH-Px) activities, the weight, childbearing and livability were examined.

RESULTS

There were durative lower Se concentrations in serum and lower GSH-Px activities in blood, and the weight, childbearing and livability reduced in Se- group and Se-I- group from F0 to F3.

CONCLUSION

We have established prolonged selenium deficiency of the SD rat animal model breed third generation in series successfully.

摘要

目的

建立连续三代的缺硒品系SD大鼠动物模型。

方法

采用以缺硒酵母为主的缺硒饲料。将六只雌性和六只雄性SD大鼠原代（F0）分为五组，分别给予以下五种饲料：（1）硒和碘充足对照组（Se+I+组。硒：0.1 - 0.3微克/克，碘≥0.2微克/克），（2）缺硒碘充足组1（Se-I+组。硒：0.02微克/克，碘≥0.2微克/克），（3）缺硒碘充足组2（Se-I+组2。硒：0.005微克/克，碘≥0.2微克/克），（4）硒充足碘缺乏组（Se+I-组。硒：0.1 - 0.3微克/克，碘：0.04微克/克），（5）硒和碘缺乏组（Se-I-组。硒：0.01微克/克，碘：0.04微克/克）。缺硒碘充足组2从第一代（F1）开始剔除。检测血清硒和甲状腺激素浓度、全血谷胱甘肽过氧化物酶（GSH-Px）活性、体重、繁殖力和存活率。