Kralik A, Eder K, Kirchgessner M
Institute of Nutrition Physiology, Technical University Munich, Freising-Weihenstephan, Germany.
Horm Metab Res. 1996 May;28(5):223-6. doi: 10.1055/s-2007-979169.
48 weaned male Sprague-Dawley rats with an initial average body weight of 41 g were divided into 4 groups of 12 animals (zinc-deficient; zinc-adequate, pair-fed with zinc-deficient group; selenium-deficient; selenium-adequate) for 40 days. All groups were fed a semisynthetic diet with casein being the source of protein. In the selenium-deficient diet, there was a selenium concentration of 0.038 mg/kg. The other diets were supplemented with Na-selenite in order to adjust the selenium concentration to 0.3 mg/kg. In the zinc-deficient diet, there was a zinc concentration of 4.1 mg/kg. The zinc concentrations in the other diets were adjusted to 45 mg/kg by the addition of zinc-sulfate heptahydrate. Zinc-deficient rats were characterized by a markedly reduced alkaline phosphatase activity in their serum, whilst selenium-deficient rats showed a markedly reduced glutathione peroxidase in serum proving their respective zinc-deficient and selenium-deficient states. Zinc deficiency decreased concentrations of triiodothyronine (T3) and free thyroxine (fT4) in serum by approximately 30% when compared with zinc-adequate controls. The concentration of thyroxine (T4) in serum was not affected by zinc deficiency. Selenium-deficient animals had lower concentrations of T3 and T4 than selenium-adequate animals. The concentration of fT4 in serum was not affected by selenium deficiency. The activity of hepatic type I 5'deiodinase was decreased by 67% by zinc deficiency and by 47% by selenium deficiency compared to adequate controls. The study data show that both zinc and selenium deficiency affect the metabolism of thyroid hormones.
48只断奶雄性Sprague-Dawley大鼠，初始平均体重为41克，被分为4组，每组12只动物（缺锌组；锌充足组，与缺锌组配对喂养；缺硒组；硒充足组），为期40天。所有组均喂食以酪蛋白为蛋白质来源的半合成饮食。在缺硒饮食中，硒浓度为0.038毫克/千克。其他饮食添加亚硒酸钠，以便将硒浓度调整至0.3毫克/千克。在缺锌饮食中，锌浓度为4.1毫克/千克。通过添加七水硫酸锌将其他饮食中的锌浓度调整至45毫克/千克。缺锌大鼠的特征是血清碱性磷酸酶活性显著降低，而缺硒大鼠血清中的谷胱甘肽过氧化物酶显著降低，证明了它们各自的缺锌和缺硒状态。与锌充足的对照组相比，缺锌使血清中三碘甲状腺原氨酸（T3）和游离甲状腺素（fT4）的浓度降低了约30%。血清中甲状腺素（T4）的浓度不受缺锌影响。缺硒动物的T3和T4浓度低于硒充足的动物。血清中fT4的浓度不受缺硒影响。与充足对照组相比，缺锌使肝脏I型5'脱碘酶的活性降低了67%，缺硒使其降低了47%。研究数据表明，缺锌和缺硒均会影响甲状腺激素的代谢。
