Lombard-Vignon N, Grizard G, Boucher D
Laboratoire de Biologie de la Reproduction et du Développement, Faculté de Médecine, Clermont-Ferrand, France.
Int J Androl. 1992 Apr;15(2):144-59. doi: 10.1111/j.1365-2605.1992.tb01123.x.
The influence of co-cultures of rat testicular macrophages and Leydig cells (LC) on LC morphology and steroidogenesis was investigated with and without macrophage stimulation by a bacterial lipopolysaccharide (LPS). LC showed an elongated form in the presence of stimulated testicular macrophages. In the presence of non-stimulated testicular macrophages a significant inhibition of testosterone production was observed (decrease of 33%) from 48 h in co-culture while an increase of 16% was obtained at the same culture time, after stimulation of macrophages by LPS. When LC were treated with testicular macrophage-conditioned media (MCM) obtained from LPS-treated macrophages, they became fusiform and there was stimulation (78%) of steroid production. After human FSH stimulation (1-1000 mIU ml-1), MCM from testicular macrophages was no more effective in enhancing testosterone production by LC than was media from untreated LC. Similar experiments with LPS were conducted with macrophages of peritoneal origin. Peritoneal macrophages stimulated or not by LPS in co-cultures with LC or peritoneal MCM did not significantly modify testosterone production. However, these cells were able to modify LC morphology when LPS-MCM was added to LC-culture medium. The present results suggest strongly that testicular macrophage-LC interactions could be important in the control of LC steroidogenesis.
研究了大鼠睾丸巨噬细胞与睾丸间质细胞（LC）共培养，以及细菌脂多糖（LPS）刺激巨噬细胞与否对LC形态和类固醇生成的影响。在存在受刺激的睾丸巨噬细胞时，LC呈现出细长的形态。在存在未受刺激的睾丸巨噬细胞时，共培养48小时后观察到睾酮生成受到显著抑制（降低33%），而在用LPS刺激巨噬细胞后，在相同培养时间获得了16%的增加。当用从经LPS处理的巨噬细胞获得的睾丸巨噬细胞条件培养基（MCM）处理LC时，它们变成梭形，并且类固醇生成受到刺激（增加78%）。在人促卵泡激素（FSH）刺激（1 - 1000 mIU/ml）后，来自睾丸巨噬细胞的MCM在增强LC睾酮生成方面并不比未处理的LC培养基更有效。用源自腹膜的巨噬细胞进行了类似的LPS实验。在与LC共培养或加入腹膜MCM时，无论是否用LPS刺激的腹膜巨噬细胞都不会显著改变睾酮生成。然而，当将LPS - MCM添加到LC培养基中时，这些细胞能够改变LC的形态。目前的结果强烈表明，睾丸巨噬细胞与LC的相互作用在控制LC类固醇生成中可能很重要。
