Yee J B, Hutson J C
Endocrinology. 1985 Jun;116(6):2682-4. doi: 10.1210/endo-116-6-2682.
Conditioned medium from cultures of testicular macrophages was capable of stimulating testosterone production in a dose-dependent manner when added to Leydig cells in vitro. Significant stimulation of testosterone production by Leydig cells was observed after 4 h of exposure to testicular macrophage-conditioned medium (TMCM) and thereafter increased progressively for up to 24 h. Treatment of Leydig cells with TMCM together with a maximal dose of LH resulted in greater production of testosterone by Leydig cells than with either agent when used separately. Conditioned medium from macrophages treated with FSH was twice as potent as TMCM from untreated cells. This dose of FSH had no direct effect on Leydig cells. Conditioned medium from cultures of peritoneal macrophages had less effect on testosterone production by Leydig cells than testicular macrophage-conditioned medium. It is proposed that secretory products from testicular macrophages play an important role in testicular function.
当体外添加到睾丸间质细胞时，睾丸巨噬细胞培养的条件培养基能够以剂量依赖的方式刺激睾酮的产生。在暴露于睾丸巨噬细胞条件培养基（TMCM）4小时后，观察到睾丸间质细胞睾酮产生受到显著刺激，此后直至24小时逐渐增加。用TMCM和最大剂量的LH共同处理睾丸间质细胞，与单独使用任何一种试剂相比，睾丸间质细胞产生的睾酮更多。用FSH处理的巨噬细胞的条件培养基的效力是未处理细胞的TMCM的两倍。该剂量的FSH对睾丸间质细胞没有直接影响。腹膜巨噬细胞培养的条件培养基对睾丸间质细胞睾酮产生的影响小于睾丸巨噬细胞条件培养基。有人提出，睾丸巨噬细胞的分泌产物在睾丸功能中起重要作用。
