Weekley R, Klein R
Children's Hospital Medical Center of Akron, Ohio.
J Burn Care Rehabil. 1992 Jan-Feb;13(1):138-41. doi: 10.1097/00004630-199201000-00031.
In 1989, the Regional Burn Center of the Children's Hospital Medical Center of Akron, Ohio, switched to use of commercially produced cultured epidermal autograft (CEA) from cultured cells that had been grown in the institution's own laboratories. CEA has the advantage of producing the quantity of grafts that are needed from the original biopsy specimen. Clinical experience with 10 patients has demonstrated an average of 72% "take" with approximately 1309 grafts. Arms, legs, and anterior trunk are the preferred sites for coverage. Patient/family education is an integral component of the CEA regimen; a structured educational program begins immediately. The cooperation of both patient and family is often a critical factor in successful grafting. Usually one to two biopsy specimens from the groin or the axilla are sent for culturing. The patient receives standard burn care while cultured tissue is grown, which includes physical therapy and splinting. Early excision, temporary coverage with cadaver skin, topical antibiotics, graft protection, and pain control are keys to success. Physical therapy and splinting are delayed until after removal of the graft backing, which usually occurs 10 to 12 days after CEA is applied.
1989年，俄亥俄州阿克伦市儿童医院医疗中心的地区烧伤中心转而使用由该机构自己实验室培养的细胞制成的商业生产的培养表皮自体移植物（CEA）。CEA的优点是能够从原始活检标本中生产出所需数量的移植物。对10名患者的临床经验表明，平均约1309块移植物的“成活率”为72%。手臂、腿部和前躯干是首选的覆盖部位。患者/家庭教育是CEA治疗方案不可或缺的一部分；一个结构化的教育计划立即开始。患者和家属的合作往往是成功移植的关键因素。通常从腹股沟或腋窝采集一到两个活检标本送去培养。在培养组织生长期间，患者接受标准的烧伤护理，包括物理治疗和夹板固定。早期切除、用尸体皮肤临时覆盖、局部使用抗生素、移植物保护和疼痛控制是成功的关键。物理治疗和夹板固定推迟到去除移植物衬背之后进行，这通常在应用CEA后10至12天发生。
