基于使用重组犬弓首蛔虫抗原的血清学检测法对巴西东北部弓首蛔虫病流行情况的研究
Prevalence of toxocariasis in northeastern Brazil based on serology using recombinant Toxocara canis antigen.
作者信息
De Andrade Lima Coêlho Raquel, De Carvalho Luiz Bezerra, Perez Emília Pessoa, Araki Kunioki, Takeuchi Tsutomu, Ito Akira, Aoki Takashi, Yamasaki Hiroshi
机构信息
Departamento de Bioquímica, Laboratório de Imunopatología Keizo Asami, Universidade Federal de Pernambuco, Brazil.
出版信息
Am J Trop Med Hyg. 2005 Jan;72(1):103-7.
To evaluate the prevalence of toxocariasis in children in Jaboatão dos Guararapes, Pernambuco in northeastern Brazil, 215 serum samples were examined by an enzyme-linked immunosorbent assay (ELISA) using a recombinant Toxocara canis antigen. In the ELISA, 26 (12.1%) of 215 subjects were positive. In a dot-blot assay using 53 of 215 serum samples, the diagnostic results correlated with those obtained by the ELISA. Moreover, it has been confirmed that the recombinant T. canis antigen was highly specific for toxocariasis by ELISA using serum samples positive for antibody to Ascaris lumbricoides. Considering the specificity of the recombinant antigen to toxocariasis, the ELISA or dot-blot assay using the recombinant T. canis antigen is recommended in tropical and sub-tropical regions where various parasitic infections are commonly endemic.
为评估巴西东北部伯南布哥州雅博阿唐-杜瓜拉鲁佩斯儿童的弓蛔虫病患病率，使用重组犬弓首蛔虫抗原通过酶联免疫吸附测定（ELISA）检测了215份血清样本。在ELISA检测中，215名受试者中有26名（12.1%）呈阳性。在使用215份血清样本中的53份进行的斑点印迹试验中，诊断结果与ELISA检测结果相关。此外，通过使用对蛔虫抗体呈阳性的血清样本进行ELISA检测，已证实重组犬弓首蛔虫抗原对弓蛔虫病具有高度特异性。考虑到重组抗原对弓蛔虫病的特异性，建议在各种寄生虫感染普遍流行的热带和亚热带地区使用重组犬弓首蛔虫抗原进行ELISA或斑点印迹试验。
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