Sato Megumi, Sato Marcello Otake, Waikagul Jitra, Pongvongsa Tiengkham, Sanguankiat Surapol, Yoonuan Tipparayat, Kounnavong Sengchanh, Kawai Satoru, Yamasaki Hiroshi, Moji Kazuhiko
Graduate School of Health Sciences, Niigata University, Niigata, Niigata, Japan.
Department of Tropical Medicine and Parasitology, Dokkyo Medical University, Mibu, Japan.
One Health. 2020 Oct 27;11:100191. doi: 10.1016/j.onehlt.2020.100191. eCollection 2020 Dec 20.
Toxocariasis is a zoonotic helminthiasis caused by the migrating larvae of and , common roundworms of dogs and cats. Our previous study in Savannakhet Province of Lao PDR showed an infection rate of 44.1% of spp. in dogs. Thus, we investigate if this previous high prevalence in the definitive hosts influenced the occurrence of human toxocariasis. For that we used a 38 kDa recombinant protein derived from larvae excretion secretion products in ELISA. Human serum samples were collected in the Lahanam area of Savannakhet province. The population attending the study in Lahanam village were aged from 7 to 59 years old (y/o) 65.9% (54/82) were male and 34.1% (28/82) were female. The total percentage of seropositivity to sp. was 30.4% (25/82). Males were more likely to test positive for toxocariasis with a risk ratio of 2.70 (CI95 0.87-4.93). No significant differences between ages were seen. However, it was possible to observe an increase of optical density (OD) values in ELISA according to age. The awareness of the health system on the high prevalence of seropositivity to sp. in Savannakhet can prevent irreversible consequences as permanent vision loss and seizures caused by this silent chronic disease revealed in the Lahanam area.
弓首蛔虫病是一种人畜共患的蠕虫病，由犬弓首蛔虫和猫弓首蛔虫（狗和猫的常见蛔虫）的移行幼虫引起。我们之前在老挝人民民主共和国沙湾拿吉省的研究表明，犬弓首蛔虫属的感染率为44.1%。因此，我们调查了终末宿主中先前的高流行率是否会影响人类弓首蛔虫病的发生。为此，我们在酶联免疫吸附测定（ELISA）中使用了一种源自犬弓首蛔虫幼虫排泄分泌产物的38 kDa重组蛋白。人类血清样本在沙湾拿吉省拉哈南地区采集。参与拉哈南村研究的人群年龄在7至59岁之间，65.9%（54/82）为男性，34.1%（28/82）为女性。对犬弓首蛔虫属血清反应阳性的总百分比为30.4%（25/82）。男性感染弓首蛔虫病呈阳性的可能性更大，风险比为2.70（95%置信区间0.87 - 4.93）。未观察到年龄之间的显著差异。然而，有可能观察到ELISA中的光密度（OD）值随年龄增加。沙湾拿吉卫生系统对犬弓首蛔虫属血清反应阳性高流行率的认识，可以预防拉哈南地区所揭示的这种隐匿性慢性病导致的不可逆转后果，如永久性视力丧失和癫痫发作。
