Nomura Masayoshi, Tsutsui Masato, Shimokawa Hiroaki, Fujimoto Naohiro, Ueta Yoichi, Morishita Tsuyoshi, Yanagihara Nobuyuki, Matsumoto Tetsuro
Department of Urology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
Neuroreport. 2005 Mar 15;16(4):413-7. doi: 10.1097/00001756-200503150-00021.
The effects of neuronal, endothelial, or inducible nitric oxide synthase gene disruption on the expression of oxytocin and vasopressin gene were examined in the hypothalamus (paraventricular, supraoptic, suprachiasmatic, and anterior commissural nuclei) and extrahypothalamus (bed nucleus of the stria terminalis). The oxytocin messenger RNA levels in the anterior commissural nucleus of neuronal nitric oxide synthase knockout mice were significantly higher than in control mice, but not in endothelial or inducible nitric oxide synthase knockout mice. In contrast, no significant effects of neuronal, endothelial, or inducible nitric oxide synthase gene disruption on oxytocin and vasopressin messenger RNA levels in the other hypothalamic and extrahypothalamic nuclei were observed. These results suggest that neuronal nitric-oxide-synthase-derived nitric oxide may be involved in the regulation of oxytocin gene expression in the anterior commissural nucleus.
研究了神经元型、内皮型或诱导型一氧化氮合酶基因敲除对下丘脑（室旁核、视上核、视交叉上核和前连合核）和下丘脑外（终纹床核）中催产素和加压素基因表达的影响。神经元型一氧化氮合酶基因敲除小鼠前连合核中的催产素信使核糖核酸水平显著高于对照小鼠，但在内皮型或诱导型一氧化氮合酶基因敲除小鼠中则不然。相反，未观察到神经元型、内皮型或诱导型一氧化氮合酶基因敲除对其他下丘脑和下丘脑外核中催产素和加压素信使核糖核酸水平有显著影响。这些结果表明，神经元型一氧化氮合酶衍生的一氧化氮可能参与前连合核中催产素基因表达的调节。
