Tan Qian, Zou Zhong-tao, Ning Guan-sen, Lin Zi-hao, Zhou Hong-reng, Liang Zhi-wei, Chen Xi, Wu Jian-ming
Department of Plastic Surgery, Gulou Hospital, The Medical School of Nanjing University, Nanjing 210008, P.R. China.
Zhonghua Shao Shang Za Zhi. 2004 Dec;20(6):354-6.
To establish a new method for the preparation of porcine acellular dermal matrix.
The antigenicity of the porcine dermis was weakened by removing epidermal and dermal cells from the porcine skin through the digestion with low-concentration trypsin and repeated freeze-thaw cycles. Split thickness porcine skin was treated with 0.05% trypsin to remove the cells from the epidermis and dermis. Repeated freeze-thaw cycles were employed to further weed out the residual cells within the dermis. The prepared acellular dermis was then examined grossly, as well as histologically, and also by immunohistochemical method.
No cell could be identified in the prepared porcine acellular dermal matrix. The integral basement membrane was preserved on the surface of dermal matrix with compact dermal matrix collagen structure.
Low concentration trypsinization and repeated freeze-thaw cycles seemed to be a simple and effective method for the preparation of xenogeneic acellular dermal matrix.
建立一种制备猪脱细胞真皮基质的新方法。
通过用低浓度胰蛋白酶消化并反复冻融循环，从猪皮中去除表皮和真皮细胞，以减弱猪真皮的抗原性。取猪断层皮片，用0.05%胰蛋白酶处理以去除表皮和真皮中的细胞。采用反复冻融循环进一步清除真皮内残留的细胞。然后对制备的脱细胞真皮进行大体观察、组织学检查及免疫组织化学检测。
制备的猪脱细胞真皮基质中未发现细胞。真皮基质表面完整的基底膜得以保留，真皮基质胶原结构紧密。
低浓度胰蛋白酶处理及反复冻融循环似乎是制备异种脱细胞真皮基质的一种简单有效的方法。
