Zhao Jing-Yu, Chai Jia-Ke, Song Hui-Feng, Sun Tian-Jun, Li Dong-Jie, Liu Ling-Ying, Gao Quan-Wen, Liang Li-Ming
Department of Burns & Plastic Surgery, First Affiliated Hospital of General Hospital of PLA, Beijing 100048, China.
Zhonghua Yi Xue Za Zhi. 2011 May 17;91(18):1276-80.
To investigate the changes of collagens I and III after the addition of hyaluronic acid in the transplantation of porcine acellular dermal matrix.
Full-thickness skin defects were created on the dorsa of Japanese white rabbits. And the rabbits were divided randomly into 3 groups: Group A (hyaluronic acid, porcine acellular dermal matrix plus thin skin autografts), Group B (porcine acellular dermal matrix plus thin skin autografts) and Group C (skin autografts). Skin biopsies were performed at Day 50 post-grafting to detect the contents of collagens I and III by histological examinations, immunohistochemistry method and Western blot.
The areas of skin graft were (13.3 ± 1.2), (9.5 ± 0.9) and (10.0 ± 1.4) cm(2) in Groups A, B and C respectively. Group A was larger than Groups B and C(all P < 0. 01). There was no statistical difference between Groups B and C (P > 0.05). The expressions of collagen I were 1894 ± 164, 515 ± 38 and 395 ± 43 in Groups A, B and C respectively. Group A was higher than Groups B and C (P < 0.01). And the expressions of collagen III were 5411 ± 435, 874 ± 70 and 2078 ± 175 in Groups A, B and C respectively. Group C was higher than Group B and yet lower than Group A (all P < 0.01). The ratios of collagen I and collagen III in Group A (0.39) and Group B (0.59) were higher than that of Group C (0.19) (all P < 0.01).
The addition of hyaluronic acid may boost the expression of collagens I and III and decrease the ratio of collagen I/collagen III. Thus it facilitates wound healing and basilar membrane remodeling and alleviates the contraction of skin transplant.
研究在猪脱细胞真皮基质移植中添加透明质酸后Ⅰ型和Ⅲ型胶原蛋白的变化。
在日本大白兔背部制作全层皮肤缺损。将兔子随机分为3组：A组（透明质酸、猪脱细胞真皮基质加自体薄皮片）、B组（猪脱细胞真皮基质加自体薄皮片）和C组（自体皮片）。在移植后第50天进行皮肤活检，通过组织学检查、免疫组织化学方法和蛋白质印迹法检测Ⅰ型和Ⅲ型胶原蛋白的含量。
A、B、C组皮片面积分别为(13.3±1.2)、(9.5±0.9)和(10.0±1.4)cm²。A组大于B组和C组（均P<0.01）。B组和C组之间无统计学差异（P>0.05）。A、B、C组Ⅰ型胶原蛋白表达分别为1894±164、515±38和395±43。A组高于B组和C组（P<0.01）。A、B、C组Ⅲ型胶原蛋白表达分别为5411±435、874±70和2078±175。C组高于B组但低于A组（均P<0.01）。A组（0.39）和B组（0.59）的Ⅰ型与Ⅲ型胶原蛋白比值高于C组（0.19）（均P<0.01）。
添加透明质酸可能会促进Ⅰ型和Ⅲ型胶原蛋白的表达，降低Ⅰ型/Ⅲ型胶原蛋白比值。从而促进伤口愈合和基底膜重塑，减轻皮肤移植后的收缩。
