Han Yue-qin, Chen Li-jun, Sun Xiao-jing, Zhao Guo-fa, Cheng Xiu-ying
Shandong Provincial Hospital, Shandong University, Jinan 252000, China.
Zhonghua Xue Ye Xue Za Zhi. 2004 Dec;25(12):740-4.
To explore the therapeutic effect of interleukin-11 (IL-11) on high-dose methotrexate (HDMTX) induced mucositis in Wistar's rats, the proliferative effect on CEM leukemia cell line and the antitumor effect on HDMTX.
Ninety-five 5-week old, 120 - 150 grams weight Wistar rats were randomly divided into five groups. Group A is normal control (n = 15), group B MTX control (n = 20), group C IL-11 pretreatment group before MTX injection (n = 20), group D (n = 20) the high dose IL-11 group (475 microg.kg(-1).d(-1)) after MTX injection, group E (n = 20) the low dose IL-11 group (150 microg.kg(-1).d(-1)) after MTX injection. All rats in group B approximately E were given 1 ml MTX intraperitoneally (100 mg/kg). Rats were killed at day 1, 3, 5, 7 after MTX injection. The mortality rates, changes of small intestine tissue morphology and ultra structure were observed. The proliferation of small intestine crypt cell was assayed by proliferating cell nuclear antigen (PCNA) immunohistochemical staining. MTT method was used to detect the proliferation of CEM cell line.
IL-11 treatment resulted in a significant increase of survival of HDMTX treated rats, increased of small intestinal villus length and villus/crypt ratio. IL-11 administration was associated with enhancement of small intestine mucosa recovery after HDMTX therapy. Group C showed a greater effect than group B (P < 0.01). IL-11 had no effect on CEM cell proliferation.
IL-11 has a significant mitigating effect on high-dose MTX induced intestinal mucositis in rat, and significantly increase the survival of the rats. IL-11 could be safely used in the HDMTX treatment of childhood acute lymphocyte leukemia.
探讨白细胞介素-11（IL-11）对大剂量甲氨蝶呤（HDMTX）诱导的Wistar大鼠黏膜炎的治疗作用、对CEM白血病细胞系的增殖作用以及对HDMTX的抗肿瘤作用。
将95只5周龄、体重120 - 150克的Wistar大鼠随机分为五组。A组为正常对照组（n = 15），B组为MTX对照组（n = 20），C组为MTX注射前IL-11预处理组（n = 20），D组为MTX注射后高剂量IL-11组（475微克·千克⁻¹·天⁻¹）（n = 20），E组为MTX注射后低剂量IL-11组（150微克·千克⁻¹·天⁻¹）（n = 20）。B组至E组的所有大鼠均腹腔注射1毫升MTX（100毫克/千克）。在MTX注射后第1、3、5、7天处死大鼠。观察死亡率、小肠组织形态和超微结构的变化。采用增殖细胞核抗原（PCNA）免疫组化染色检测小肠隐窝细胞的增殖情况。采用MTT法检测CEM细胞系的增殖情况。
IL-11治疗导致HDMTX处理大鼠的存活率显著提高，小肠绒毛长度和绒毛/隐窝比值增加。IL-11给药与HDMTX治疗后小肠黏膜恢复增强有关。C组的效果比B组更显著（P < 0.01）。IL-11对CEM细胞增殖无影响。
IL-11对大剂量MTX诱导的大鼠肠道黏膜炎具有显著的缓解作用，并显著提高大鼠的存活率。IL-11可安全用于儿童急性淋巴细胞白血病的HDMTX治疗。
