Shen Min, Zeng Xue-jun, Tang Fu-lin
Department of Rheumatism and Immunology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
Zhonghua Nei Ke Za Zhi. 2004 Dec;43(12):928-31.
To investigate the expression of human cartilage glycoprotein 39 (HC gp-39) in peripheral blood mononuclear cells (PBMC) and synovium of rheumatoid arthritis (RA) patients.
Levels of HC gp-39 mRNA were detected by reverse transcription-polymerase chain reaction in PBMC of 31 patients with RA, 6 with osteoarthritis (OA), 10 with spondylarthropathy (SpA), 5 with systemic lupus erythematosus (SLE), and of 10 healthy controls. Levels of HC gp-39 mRNA were also detected in synovium of 7 patients with RA and 5 with OA. The expression of HC gp-39 was semi-quatificated by HC gp-39/tubulin ratio.
HC gp-39 mRNA expression in PBMC was increased in RA patients (the HC gp-39/tubulin ratio was 0.8690 +/- 0.5240), compared with OA (P = 0.024), SpA (P = 0.049), SLE (P = 0.043) and with healthy control subjects (P = 0.033). There were no statistically significant differences among OA, SpA, SLE and healthy controls. The level of HC gp-39 mRNA expression in RA synovium was also significantly higher than the level found in OA (P = 0.04).
HC gp-39 mRNA was obviously overrepresented in RA patients PBMC and synovium. These data support a possible pathogenic role of HC gp-39, as a candidate autoantigen, in the autoimmune response of RA.
研究人软骨糖蛋白39（HC gp - 39）在类风湿关节炎（RA）患者外周血单个核细胞（PBMC）及滑膜中的表达。
采用逆转录聚合酶链反应检测31例RA患者、6例骨关节炎（OA）患者、10例脊柱关节病（SpA）患者、5例系统性红斑狼疮（SLE）患者及10名健康对照者PBMC中HC gp - 39 mRNA水平。同时检测7例RA患者及5例OA患者滑膜中HC gp - 39 mRNA水平。通过HC gp - 39/微管蛋白比值对HC gp - 39表达进行半定量分析。
与OA患者（P = 0.024）、SpA患者（P = 0.049）、SLE患者（P = 0.043）及健康对照者（P = 0.033）相比，RA患者PBMC中HC gp - 39 mRNA表达升高。OA患者、SpA患者、SLE患者及健康对照者之间无统计学显著差异。RA滑膜中HC gp - 39 mRNA表达水平也显著高于OA患者（P = 0.04）。
HC gp - 39 mRNA在RA患者的PBMC及滑膜中明显高表达。这些数据支持HC gp - 39作为候选自身抗原在RA自身免疫反应中可能的致病作用。
