类风湿关节炎患者外周血及滑膜单核细胞中人类软骨糖蛋白39信使核糖核酸的表达

[Human cartilage glycoprotein 39 mRNA expression in peripheral blood and synovium mononuclear cells in rheumatoid arthritis].

作者信息

Shen Min, Zeng Xue-jun, Tang Fu-lin

机构信息

Department of Rheumatism and Immunology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.

出版信息

Zhonghua Nei Ke Za Zhi. 2004 Dec;43(12):928-31.

PMID:15730740

Abstract

OBJECTIVE

To investigate the expression of human cartilage glycoprotein 39 (HC gp-39) in peripheral blood mononuclear cells (PBMC) and synovium of rheumatoid arthritis (RA) patients.

METHODS

Levels of HC gp-39 mRNA were detected by reverse transcription-polymerase chain reaction in PBMC of 31 patients with RA, 6 with osteoarthritis (OA), 10 with spondylarthropathy (SpA), 5 with systemic lupus erythematosus (SLE), and of 10 healthy controls. Levels of HC gp-39 mRNA were also detected in synovium of 7 patients with RA and 5 with OA. The expression of HC gp-39 was semi-quatificated by HC gp-39/tubulin ratio.

RESULTS

HC gp-39 mRNA expression in PBMC was increased in RA patients (the HC gp-39/tubulin ratio was 0.8690 +/- 0.5240), compared with OA (P = 0.024), SpA (P = 0.049), SLE (P = 0.043) and with healthy control subjects (P = 0.033). There were no statistically significant differences among OA, SpA, SLE and healthy controls. The level of HC gp-39 mRNA expression in RA synovium was also significantly higher than the level found in OA (P = 0.04).

CONCLUSIONS

HC gp-39 mRNA was obviously overrepresented in RA patients PBMC and synovium. These data support a possible pathogenic role of HC gp-39, as a candidate autoantigen, in the autoimmune response of RA.

摘要

目的

研究人软骨糖蛋白39（HC gp - 39）在类风湿关节炎（RA）患者外周血单个核细胞（PBMC）及滑膜中的表达。

方法

采用逆转录聚合酶链反应检测31例RA患者、6例骨关节炎（OA）患者、10例脊柱关节病（SpA）患者、5例系统性红斑狼疮（SLE）患者及10名健康对照者PBMC中HC gp - 39 mRNA水平。同时检测7例RA患者及5例OA患者滑膜中HC gp - 39 mRNA水平。通过HC gp - 39/微管蛋白比值对HC gp - 39表达进行半定量分析。

结果

与OA患者（P = 0.024）、SpA患者（P = 0.049）、SLE患者（P = 0.043）及健康对照者（P = 0.033）相比，RA患者PBMC中HC gp - 39 mRNA表达升高。OA患者、SpA患者、SLE患者及健康对照者之间无统计学显著差异。RA滑膜中HC gp - 39 mRNA表达水平也显著高于OA患者（P = 0.04）。