人外周血和滑膜中软骨gp - 39 +、CD16 +单核细胞：与类风湿关节炎关节破坏的相关性

Human cartilage gp-39+,CD16+ monocytes in peripheral blood and synovium: correlation with joint destruction in rheumatoid arthritis.

作者信息

Baeten D, Boots A M, Steenbakkers P G, Elewaut D, Bos E, Verheijden G F, Berheijden G, Miltenburg A M, Rijnders A W, Veys E M, De Keyser F

机构信息

Department of Rheumatology, Ghent University Hospital, Belgium.

出版信息

Arthritis Rheum. 2000 Jun;43(6):1233-43. doi: 10.1002/1529-0131(200006)43:6<1233::AID-ANR6>3.0.CO;2-9.

DOI:10.1002/1529-0131(200006)43:6<1233::AID-ANR6>3.0.CO;2-9

PMID:10857782

Abstract

OBJECTIVE

To investigate the expression of human cartilage (HC) gp-39, a possible autoantigen in rheumatoid arthritis (RA), in peripheral blood and synovium, to characterize its cellular source, and to analyze correlations with clinical features.

METHODS

The expression of HC gp-39 in synovium and peripheral blood mononuclear cells (PBMC) was assessed by immunohistochemistry and flow cytometry. Synthesis and secretion were investigated by both reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay.

RESULTS

PBMC expressing HC gp-39 were increased in RA patients compared with spondylarthropathy patients (P = 0.0029) and with healthy control subjects (P = 0.0013). HC gp-39+ cells were also slightly overrepresented in RA synovium (P = 0.01). In both blood and synovium, HC gp-39+ cells were identified as CD14dim,CD16+ monocytes, a phenotype which can differentiate from classic CD14++ monocytes by maturation in vitro. HC gp-39 messenger RNA was detected in RA synovium and PBMC, and PBMC secreted HC gp-39 in vitro. The number of HC gp-39+ PBMC correlated with serum levels of C-reactive protein (r = 0.39, P = 0.003) and HC gp-39 (r = 0.52, P = 0.014). HC gp-39 expression in RA synovial lining correlated with joint destruction (r = 0.77, P < 0.001).

CONCLUSION

CD16+ monocytes, a cellular source of HC gp-39 in vivo, are overrepresented in both RA peripheral blood and synovial tissue. The presence of HC gp-39+ cells in RA synovium is correlated with the degree of joint destruction. These data support a role of these cells in the local autoimmune response that leads to chronic inflammation and joint destruction.

摘要

目的

研究人类软骨（HC）gp - 39（类风湿关节炎（RA）中一种可能的自身抗原）在外周血和滑膜中的表达，确定其细胞来源，并分析其与临床特征的相关性。

方法

采用免疫组织化学和流式细胞术评估滑膜和外周血单个核细胞（PBMC）中HC gp - 39的表达。通过逆转录 - 聚合酶链反应和酶联免疫吸附测定研究其合成与分泌。

结果

与脊柱关节病患者（P = 0.0029）和健康对照者（P = 0.0013）相比，RA患者中表达HC gp - 39的PBMC增多。HC gp - 39 +细胞在RA滑膜中也略有增多（P = 0.01）。在血液和滑膜中，HC gp - 39 +细胞均被鉴定为CD14dim、CD16 +单核细胞，该表型可通过体外成熟从经典的CD14 ++单核细胞分化而来。在RA滑膜和PBMC中检测到HC gp - 39信使核糖核酸，且PBMC在体外分泌HC gp - 39。HC gp - 39 + PBMC的数量与血清C反应蛋白水平相关（r = 0.39，P = 0.003），与HC gp - 39相关（r = 0.52，P = 0.014）。RA滑膜衬里中HC gp - 39的表达与关节破坏相关（r = 0.77，P < 0.001）。