Baeten D, Boots A M, Steenbakkers P G, Elewaut D, Bos E, Verheijden G F, Berheijden G, Miltenburg A M, Rijnders A W, Veys E M, De Keyser F
Department of Rheumatology, Ghent University Hospital, Belgium.
Arthritis Rheum. 2000 Jun;43(6):1233-43. doi: 10.1002/1529-0131(200006)43:6<1233::AID-ANR6>3.0.CO;2-9.
To investigate the expression of human cartilage (HC) gp-39, a possible autoantigen in rheumatoid arthritis (RA), in peripheral blood and synovium, to characterize its cellular source, and to analyze correlations with clinical features.
The expression of HC gp-39 in synovium and peripheral blood mononuclear cells (PBMC) was assessed by immunohistochemistry and flow cytometry. Synthesis and secretion were investigated by both reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay.
PBMC expressing HC gp-39 were increased in RA patients compared with spondylarthropathy patients (P = 0.0029) and with healthy control subjects (P = 0.0013). HC gp-39+ cells were also slightly overrepresented in RA synovium (P = 0.01). In both blood and synovium, HC gp-39+ cells were identified as CD14dim,CD16+ monocytes, a phenotype which can differentiate from classic CD14++ monocytes by maturation in vitro. HC gp-39 messenger RNA was detected in RA synovium and PBMC, and PBMC secreted HC gp-39 in vitro. The number of HC gp-39+ PBMC correlated with serum levels of C-reactive protein (r = 0.39, P = 0.003) and HC gp-39 (r = 0.52, P = 0.014). HC gp-39 expression in RA synovial lining correlated with joint destruction (r = 0.77, P < 0.001).
CD16+ monocytes, a cellular source of HC gp-39 in vivo, are overrepresented in both RA peripheral blood and synovial tissue. The presence of HC gp-39+ cells in RA synovium is correlated with the degree of joint destruction. These data support a role of these cells in the local autoimmune response that leads to chronic inflammation and joint destruction.
研究人类软骨（HC）gp - 39（类风湿关节炎（RA）中一种可能的自身抗原）在外周血和滑膜中的表达，确定其细胞来源，并分析其与临床特征的相关性。
采用免疫组织化学和流式细胞术评估滑膜和外周血单个核细胞（PBMC）中HC gp - 39的表达。通过逆转录 - 聚合酶链反应和酶联免疫吸附测定研究其合成与分泌。
与脊柱关节病患者（P = 0.0029）和健康对照者（P = 0.0013）相比，RA患者中表达HC gp - 39的PBMC增多。HC gp - 39 +细胞在RA滑膜中也略有增多（P = 0.01）。在血液和滑膜中，HC gp - 39 +细胞均被鉴定为CD14dim、CD16 +单核细胞，该表型可通过体外成熟从经典的CD14 ++单核细胞分化而来。在RA滑膜和PBMC中检测到HC gp - 39信使核糖核酸，且PBMC在体外分泌HC gp - 39。HC gp - 39 + PBMC的数量与血清C反应蛋白水平相关（r = 0.39，P = 0.003），与HC gp - 39相关（r = 0.52，P = 0.014）。RA滑膜衬里中HC gp - 39的表达与关节破坏相关（r = 0.77，P < 0.001）。
CD16 +单核细胞是体内HC gp - 39的细胞来源，在RA外周血和滑膜组织中均增多。RA滑膜中HC gp - 39 +细胞的存在与关节破坏程度相关。这些数据支持这些细胞在导致慢性炎症和关节破坏的局部自身免疫反应中起作用。
