Narita T, Numao H
Department of Pathology, Hirosaki University School of Medicine, Japan.
J Histochem Cytochem. 1992 May;40(5):681-7. doi: 10.1177/40.5.1573249.
We investigated lectin binding patterns on tissue specimens of normal and metaplastic gastric surface mucosae, gastric adenomas, and intestinal and diffuse-type gastric carcinomas. Compared with normal gastric mucosa, metaplastic mucosa exhibited an increase of ConA binding and decreases of WGA, PNA, UEA-1, and DBA binding in the cytoplasm, and decreases of ConA, PNA, and UEA-1 binding at the luminal surface. Intestinal carcinomas were similar to metaplastic gastric surface mucosa in ConA, WGA, and UEA-1 binding in the cytoplasm, while diffuse-type carcinomas were similar to normal gastric mucosa in WGA and UEA-1 binding in the cytoplasm. Adenomas were similar to intestinal carcinomas in ConA and UEA-1 binding in the cytoplasm, but were different from intestinal carcinomas in Con A and UEA-1 binding at the luminal surface. For UEA-1, normal and metaplastic gastric surface mucosae did not show a significant difference between the blood type A, AB, B group and the O group. Intestinal and diffuse carcinomas and adenomas also did not show such a difference between the blood groups. For DBA, normal gastric surface mucosa showed a significant difference between the blood type B, O group and the A, AB group. Normal gastric mucosa of the blood type A, AB group was frequently positive for DBA binding in the cytoplasm and at the luminal surface. Metaplastic mucosa did not show a significant difference between the blood groups. Intestinal and diffuse-type carcinomas and adenomas also did not show a difference between the blood groups. DBA binding in the cytoplasm of intestinal carcinomas and adenomas was more frequently positive than that of normal and metaplastic mucosae, except for normal gastric mucosa of the blood type A, AB group. Compared with diffuse-type carcinomas, intestinal carcinomas were accompanied by a significant increase of ConA binding and decreases of WGA and PNA binding in the cytoplasm.
我们研究了正常和化生的胃表面黏膜、胃腺瘤以及肠型和弥漫型胃癌组织标本上的凝集素结合模式。与正常胃黏膜相比，化生黏膜在细胞质中伴刀豆球蛋白A（ConA）结合增加，小麦胚凝集素（WGA）、花生凝集素（PNA）、荆豆凝集素-1（UEA-1）和双花扁豆凝集素（DBA）结合减少，在管腔表面ConA、PNA和UEA-1结合减少。肠型癌在细胞质中ConA、WGA和UEA-1结合方面与化生的胃表面黏膜相似，而弥漫型癌在细胞质中WGA和UEA-1结合方面与正常胃黏膜相似。腺瘤在细胞质中ConA和UEA-1结合方面与肠型癌相似，但在管腔表面ConA和UEA-1结合方面与肠型癌不同。对于UEA-1，正常和化生的胃表面黏膜在A型、AB型、B型血组与O型血组之间未显示出显著差异。肠型癌、弥漫型癌和腺瘤在血型组之间也未显示出这种差异。对于DBA，正常胃表面黏膜在B型、O型血组与A型、AB型血组之间显示出显著差异。A型、AB型血组的正常胃黏膜在细胞质和管腔表面DBA结合方面常呈阳性。化生黏膜在血型组之间未显示出显著差异。肠型癌、弥漫型癌和腺瘤在血型组之间也未显示出差异。除A型、AB型血组的正常胃黏膜外，肠型癌和腺瘤细胞质中的DBA结合比正常和化生黏膜更常呈阳性。与弥漫型癌相比，肠型癌在细胞质中伴有ConA结合显著增加，WGA和PNA结合减少。
