Kuhlmann W D, Peschke P, Wurster K
Virchows Arch A Pathol Anat Histopathol. 1983;398(3):319-28. doi: 10.1007/BF00583588.
Peroxidase (HRP) conjugates of Dolichos biflorus agglutinin (DBA), Arachis hypogaea agglutinin (PNA) and Ulex europaeus agglutinin I (UEA I) were used as specific molecular probes in order to describe histological patterns of carbohydrate moieties in glycoconjugates of gastric mucosa. Specimens of normal mucosa, metaplasias and carcinomas were fixed, embedded in paraffin and stained with lectins by a postembedding technique. In normal mucosa, DBA reacted with surface epithelial, parietal and antral gland cells; neck cells did not react. In single goblet cell metaplasia, with few exceptions, goblet cells showed DBA binding. In contrast, mainly negative goblet cells were seen in intestinal metaplasia. In gastric carcinomas of intestinal or diffuse types, heterogeneous staining patterns were observed. Large numbers of mucus-producing surface epithelial, neck and antral gland cells stained with PNA-HRP conjugates. Parietal cells did not react. Among the chief cells only few showed PNA binding. In single goblet cell metaplasia, equal numbers of goblet cells were positive or negative, whereas goblet cells in intestinal metaplasia were mainly unstained. Carcinomas contained both PNA-positive and PNA-negative cells; PNA-positive cells occurred preferentially in the tumor periphery. UEA I - HRP conjugates stained the bulk of surface epithelial and neck cells; chief cells and antral gland cells were mainly positive, too. Parietal cells did not react. In single goblet cell metaplasia and intestinal metaplasia, UEA I-positive as well as UEA-negative goblet cells occurred. Striated cells exhibited a great variety of lectin reactivity. Carcinomas also showed a heterogeneous staining pattern. Under both normal conditions and in carcinoma development, qualitative and quantitative differences in the composition of glycoconjugates must be expected.
为描述胃黏膜糖缀合物中碳水化合物部分的组织学模式，将双花扁豆凝集素（DBA）、花生凝集素（PNA）和欧洲荆豆凝集素I（UEA I）的过氧化物酶（HRP）缀合物用作特异性分子探针。取正常黏膜、化生及癌的标本进行固定，石蜡包埋，并采用包埋后技术用凝集素染色。在正常黏膜中，DBA与表面上皮细胞、壁细胞和胃窦腺细胞发生反应；颈部细胞无反应。在单杯状细胞化生中，除少数例外，杯状细胞显示DBA结合。相反，在肠化生中主要可见阴性杯状细胞。在肠型或弥漫型胃癌中，观察到异质性染色模式。大量产生黏液的表面上皮细胞、颈部细胞和胃窦腺细胞被PNA-HRP缀合物染色。壁细胞无反应。在主细胞中，只有少数显示PNA结合。在单杯状细胞化生中，杯状细胞阳性和阴性的数量相等，而肠化生中的杯状细胞主要未染色。癌组织中既有PNA阳性细胞，也有PNA阴性细胞；PNA阳性细胞优先出现在肿瘤周边。UEA I-HRP缀合物使大部分表面上皮细胞和颈部细胞染色；主细胞和胃窦腺细胞也主要呈阳性。壁细胞无反应。在单杯状细胞化生和肠化生中，出现了UEA I阳性和UEA阴性杯状细胞。纹状细胞表现出多种凝集素反应性。癌组织也显示出异质性染色模式。在正常情况和癌发展过程中，预计糖缀合物组成会存在定性和定量差异。
