[免疫亲和柱及高效液相色谱法测定谷物中的赭曲霉毒素A。第二部分。乙腈/水萃取法的评估]

[Immunoaffinity columns and determination of ochratoxin A in cereals by HPLC. Part II. Evaluation of extraction using acetonitrile/water].

作者信息

Czerwiecki Ludwik, Czyzyk Kamila, Wilczyńska Grazyna, Kwiecień Agnieszka

机构信息

Zakład Analizy Zywności, Instytut Biotechnologii Przemysłu Rolno-Spozywczego, 02-532 Warszawa, ul. Rakowiecka 36.

出版信息

Rocz Panstw Zakl Hig. 2004;55(3):249-53.

PMID:15732503

Abstract

Ochratoxin A from wheat and rye grain was extracted with acetonitrile:water (60:40). After clean-up of extracts using immunoaffinity columns (IAC), ochratoxin was determined by high-performance liquid chromatography using C18 column and fluorometric detection at 330 nm excitation and 460 nm emission. The mean recovery of ochratoxin A at fortification levels 0.1-100 microg/kg, was 74-89%. The limits of detection (LOD) and quantification (LOQ) were 0.015 and 0.025 microg/kg, respectively. The positive results were confirmed by reaction with BF3 complex in methanol.

摘要

用乙腈

水（60:40）从小麦和黑麦谷物中提取赭曲霉毒素A。使用免疫亲和柱（IAC）对提取物进行净化后，采用C18柱，在330nm激发波长和460nm发射波长下进行荧光检测，通过高效液相色谱法测定赭曲霉毒素。在0.1 - 100μg/kg的加标水平下，赭曲霉毒素A的平均回收率为74 - 89%。检测限（LOD）和定量限（LOQ）分别为0.015和0.025μg/kg。阳性结果通过与甲醇中的BF3络合物反应进行确认。