[谷物及谷物制品中桔青霉素和赭曲霉毒素A含量的同时测定方法]

[The method of the simultaneous determination of the citrinin and ochratoxin A content in cereals and cereal products].

作者信息

Eller K I, Pimenova V V, Kiseleva M G, Perederiaev O I, Aksenov I V, Medvedev Iu V

出版信息

Vopr Pitan. 2006;75(4):53-7.

Abstract

The simplifyed method of simultaneous determination of the citrinin (CT) and ochratoxin A (OTA) in cereals is described. The extraction of mycotoxins was carried out by small volumes of solvents (water/aceton/hexane) without additional sample clean up and concentration. The extracts were analysed by HPLC using mixture of methanol/ethylacetate/phosphoric acid, pH 2.2 as a mobile phase and fluorescence detection (lamda ex 330 nm, lamda em 495 nm). The reliability and reproducibility of results were improved by usage of internal standard--methyl-derivative of ochratoxin A. Average recovery of CT and OTA was 70%, relative standard deviation 12% and 7%, respectively, limit of detection 0.003 mg/kg.

摘要

描述了同时测定谷物中桔青霉素（CT）和赭曲霉毒素A（OTA）的简化方法。霉菌毒素的提取通过使用少量溶剂（水/乙腈/己烷）进行，无需额外的样品净化和浓缩。提取物通过高效液相色谱法进行分析，使用甲醇/乙酸乙酯/磷酸（pH 2.2）的混合物作为流动相，并进行荧光检测（激发波长330 nm，发射波长495 nm）。通过使用内标——赭曲霉毒素A的甲基衍生物提高了结果的可靠性和重现性。CT和OTA的平均回收率分别为70%，相对标准偏差分别为12%和7%，检测限为0.003 mg/kg。