Bakhanashvili Mary, Novitsky Elena, Levy Itzchak, Rahav Galia
Infectious Diseases Unit, Chaim Sheba Medical Center, Tel Hashomer 52621, Israel.
FEBS Lett. 2005 Feb 28;579(6):1435-40. doi: 10.1016/j.febslet.2005.01.043.
The high error rates characteristic of human immunodeficiency virus type-1 reverse transcriptase (HIV-1 RT) are a presumptive source of the viral hypervariability that substantially affect viral pathogenesis and resistance to drug therapy. We have analyzed the potential role of polyamines in the fidelity of DNA synthesis by HIV-1 RT. The current study suggest that polyamines tested has the potential to be "antimutator". The polyamines exert the ability to reduce the misincorporation and mispair extension with both RNA/DNA and DNA/DNA template-primers in the following order: spermine > spermidine > putrescine. In view of the significance of mutations of HIV, the possible roles of polyamines in the accuracy of DNA synthesis could be of particular importance; polyamines may affect the mutation rate of the virus.
人类免疫缺陷病毒1型逆转录酶（HIV-1 RT）的高错误率是病毒高度变异性的一个推测来源，这极大地影响了病毒的发病机制和对药物治疗的抗性。我们分析了多胺在HIV-1 RT进行DNA合成保真度方面的潜在作用。当前研究表明，所测试的多胺有成为“抗突变剂”的潜力。多胺具有降低RNA/DNA和DNA/DNA模板引物错配掺入及错配延伸的能力，其作用顺序为：精胺>亚精胺>腐胺。鉴于HIV突变的重要性，多胺在DNA合成准确性方面的可能作用可能尤为重要；多胺可能会影响病毒的突变率。
