用于检测奶牛抗牛支原体抗体的单克隆阻断酶联免疫吸附测定法的开发及其与聚合酶链反应检测的比较

Development of a monoclonal blocking ELISA for the detection of antibody to Mycoplasma bovis in dairy cattle and comparison to detection by PCR.

作者信息

Ghadersohi Ali, Fayazi Zahra, Hirst Robert G

机构信息

Department Microbiology and Immunology and Australian Institute of Tropical Veterinary and Animal Science James Cook University, Townsville, Qld. 4811, Australia.

出版信息

Vet Immunol Immunopathol. 2005 Apr 8;104(3-4):183-93. doi: 10.1016/j.vetimm.2004.11.008.

DOI:10.1016/j.vetimm.2004.11.008

PMID:15734539

Abstract

A monoclonal antibody blocking enzyme-linked immunosorbent assay (B-ELISA) was developed to detect antibodies to Mycoplasma bovis in cattle sera. The assay was highly specific and sensitive and there was no cross-reaction detected. This method revealed a high prevalence of antibodies (60%) to M. bovis in dairy cattle in North Queensland. The diagnostic potential of this B-ELISA for the detection of antibody to M. bovis was compared with its detection by PCR. There was a strong positive correlation between PCR and B-ELISA titers. Thus, the B-ELISA appears to be a valuable and reproducible tool in the serodiagnosis of M. bovis infection in cattle.

摘要

开发了一种单克隆抗体阻断酶联免疫吸附测定法（B-ELISA）来检测牛血清中针对牛支原体的抗体。该测定法具有高度特异性和敏感性，未检测到交叉反应。此方法显示北昆士兰奶牛中针对牛支原体的抗体流行率很高（60%）。将这种B-ELISA检测牛支原体抗体的诊断潜力与其通过PCR检测的结果进行了比较。PCR和B-ELISA滴度之间存在很强的正相关性。因此，B-ELISA似乎是牛支原体感染血清诊断中有价值且可重复的工具。