Institute of Molecular Pathogenesis, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Jena, Germany.
Vet J. 2010 Dec;186(3):299-303. doi: 10.1016/j.tvjl.2009.10.008. Epub 2009 Nov 18.
Mycoplasma bovis infection of cattle tends to persist in affected herds and can be resistant to treatment. Given that the level of shedding of the organism can reflect the state of ongoing infection, a study was established to quantify the degree of such shedding in milk samples from infected herds using a novel real-time PCR technique. While the M. bovis load in herds with clinical disease was significantly higher than in disease-free herds (P<0.001), infection persisted in the latter. Similarly, M. bovis was detected more frequently in conjunctival swabs from calves with respiratory disease when compared with samples from animals that did not exhibit such clinical signs. This novel real-time PCR assay is specific for M. bovis and can detect bacterial loads as low as 100 colony forming units/mL of milk.
牛支原体感染牛群往往持续存在，并且可能对治疗有抵抗力。鉴于该生物体的脱落水平可以反映持续感染的状态，因此建立了一项研究，使用新型实时 PCR 技术来定量感染牛群的牛奶样本中的这种脱落程度。虽然临床疾病牛群中的 M. bovis 负荷明显高于无疾病牛群（P<0.001），但后者仍存在感染。同样，与没有表现出这种临床症状的动物的样本相比，从患有呼吸道疾病的小牛的结膜拭子中更频繁地检测到 M. bovis。这种新型实时 PCR 检测方法对 M. bovis 具有特异性，并且可以检测到低至 100 个细菌形成单位/毫升牛奶的细菌负荷。
