Engels Eric A, Chen Jinbo, Hartge Patricia, Cerhan James R, Davis Scott, Severson Richard K, Cozen Wendy, Viscidi Raphael P
Viral Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Department of Health and Human Services, 6120 Executive Boulevard, EPS 8010, Rockville, MD 20892, USA.
Cancer Epidemiol Biomarkers Prev. 2005 Feb;14(2):521-4. doi: 10.1158/1055-9965.EPI-04-0441.
A possible role for SV40, a macaque polyomavirus, in non-Hodgkin lymphoma (NHL) in humans was raised recently by the reported detection of SV40 DNA in tumor tissue. Animals with SV40-induced tumors frequently produce high-level antibodies against T antigen, the SV40 oncoprotein. In this study, we assessed whether SV40 T antibody measured in humans supported a relationship between SV40 and NHL. Subjects were sampled from a U.S. population-based case-control study of NHL, according to presence of antibodies against capsids of SV40 and BK, a related human polyomavirus (n = 85 cases, n = 95 controls). T antibody was measured by enzyme immunoassay. We also evaluated serum specimens from SV40-infected and SV40-uninfected macaques (n = 19 and n = 8, respectively), SV40-uninfected hamsters (n = 5), and hamsters with SV40-induced tumors (n = 10). Hamsters with SV40-induced tumors all produced robust SV40 T antibody [median absorbance, 0.99), whereas SV40-uninfected hamsters and macaques had much lower levels (median absorbance, 0.05 and 0.04, respectively). NHL cases, controls, and SV40-infected macaques resembled these latter two groups, generally showing only low-level T antibody (median absorbance, 0.03, 0.04, and 0.04, respectively). Overall, only five cases (6%) and five controls (5%) had T antibody responses classified as seropositive (odds ratio, 1.2; 95% confidence interval, 0.3-4.6). Interestingly, all 10 humans with T antibody responses also showed antibody responses to BK capsid. We found no association between the presence of T antibody and NHL, arguing against SV40 as a cause of NHL. Infrequent and low-level T antibody responses among humans could represent cross-reactivity to BK virus T antigen.
最近，由于在肿瘤组织中检测到猴空泡病毒40（SV40）DNA，人们提出了这种猕猴多瘤病毒在人类非霍奇金淋巴瘤（NHL）中可能发挥的作用。患有SV40诱导肿瘤的动物经常产生针对T抗原（SV40癌蛋白）的高水平抗体。在本研究中，我们评估了在人类中检测到的SV40 T抗体是否支持SV40与NHL之间的关系。根据针对SV40衣壳和BK（一种相关的人类多瘤病毒）的抗体存在情况，从一项基于美国人群的NHL病例对照研究中选取受试者（85例病例，95例对照）。通过酶免疫测定法检测T抗体。我们还评估了受SV40感染和未受SV40感染的猕猴（分别为19只和8只）、未受SV40感染的仓鼠（5只）以及患有SV40诱导肿瘤的仓鼠（10只）的血清样本。患有SV40诱导肿瘤的仓鼠均产生了强烈的SV40 T抗体（中位吸光度为0.99），而未受SV40感染的仓鼠和猕猴的水平则低得多（中位吸光度分别为0.05和0.04）。NHL病例、对照和受SV40感染的猕猴与后两组相似，通常仅显示低水平的T抗体（中位吸光度分别为0.03、0.04和0.04）。总体而言，只有5例病例（6%）和5例对照（5%）的T抗体反应被分类为血清阳性（比值比为1.2；95%置信区间为0.3 - 4.6）。有趣的是，所有10例有T抗体反应的人也显示出对BK衣壳的抗体反应。我们发现T抗体的存在与NHL之间没有关联，这表明SV40不是NHL的病因。人类中不常见且低水平的T抗体反应可能代表对BK病毒T抗原的交叉反应。
