Cao Ying, Zhou Zhichao, de Crombrugghe Benoit, Nakashima Kazuhisa, Guan Hui, Duan Xiaoping, Jia Shu-Fang, Kleinerman Eugenie S
Division of Pediatrics and Department of Molecular Genetics, University of Texas M. D. Anderson Cancer Center, Houston, Texas, USA.
Cancer Res. 2005 Feb 15;65(4):1124-8. doi: 10.1158/0008-5472.CAN-04-2128.
Osterix is a novel zinc finger-containing transcription factor that is essential for osteoblast differentiation and bone formation. We hypothesized that osterix might have a role in osteosarcoma tumor growth and metastasis. Northern blot analysis showed that the mRNA level of osterix was decreased in two mouse osteosarcoma cell lines compared with its level in normal mouse osteoblasts. Osterix expression was also decreased in three human osteosarcoma cell lines. Transfection of the osx gene into the mouse osteosarcoma cells inhibited tumor cell growth in vitro and in vivo and significantly reduced tumor incidence, tumor volume, and lung metastasis following intratibial injection. Osterix expression was also associated with decreased osteolysis. Using an in vitro migration assay, osterix suppressed the migration of tumor cells to lung extracts. These results suggest that osterix expression may play a role in osteosarcoma tumor growth and metastasis.
osterix是一种新型的含锌指转录因子，对成骨细胞分化和骨形成至关重要。我们推测osterix可能在骨肉瘤肿瘤生长和转移中发挥作用。Northern印迹分析显示，与正常小鼠成骨细胞相比，两种小鼠骨肉瘤细胞系中osterix的mRNA水平降低。在三种人骨肉瘤细胞系中，osterix表达也降低。将osx基因转染到小鼠骨肉瘤细胞中可抑制体外和体内肿瘤细胞生长，并显著降低胫骨内注射后的肿瘤发生率、肿瘤体积和肺转移。osterix表达还与骨溶解减少有关。使用体外迁移试验，osterix抑制肿瘤细胞向肺提取物的迁移。这些结果表明，osterix表达可能在骨肉瘤肿瘤生长和转移中发挥作用。
