Aso Yuji, Koga Hanako, Sashihara Toshihiro, Nagao Jun-Ichi, Kanemasa Youhei, Nakayama Jiro, Sonomoto Kenji
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Plasmid. 2005 Mar;53(2):164-78. doi: 10.1016/j.plasmid.2004.08.003.
We report the whole DNA sequence of two plasmids, pPI-1 (30.2 kb) and pPI-2 (2.8 kb). These plasmids are from Staphylococcus warneri ISK-1, which produces a lantibiotic, nukacin ISK-1. Curing of pPI-1 resulted in a loss of bactericidal activity in the culture supernatant and the host's immunity to nukacin ISK-1, suggesting that the biosynthetic genes of the bacteriocin are encoded by pPI-1. Based on the results of a homology search of each open reading flame, pPI-1 is comprised of the following four distinct regions: (1) the nukacin ISK-1 biosynthesis and immunity gene cluster, (2) the thioredoxin gene cluster, (3) the replication region, and (4) a region of Staphylococcus epidermidis ATCC 12228, highly homologous to pSE-12228-05. Gene organization in the nukacin ISK-1 biosynthesis and immunity gene cluster is different from that in other lacticin-481 type gene clusters. The features of the replication protein encoded in the replicating region are somewhat different from other staphylococcus theta-replicating plasmids. pPI-2 comprised a disinfectant resistant gene, qacC, and the whole DNA sequence showed significant similarity to those of other qacC plasmids such as pSK108, suggesting that pPI-2 belongs to the qacC plasmid group.
我们报道了两个质粒pPI - 1（30.2 kb）和pPI - 2（2.8 kb）的完整DNA序列。这些质粒来自沃氏葡萄球菌ISK - 1，该菌可产生羊毛硫抗生素nukacin ISK - 1。去除pPI - 1导致培养上清液中杀菌活性丧失以及宿主对nukacin ISK - 1的免疫性丧失，这表明细菌素的生物合成基因由pPI - 1编码。基于对每个开放阅读框的同源性搜索结果，pPI - 1由以下四个不同区域组成：（1）nukacin ISK - 1生物合成和免疫基因簇；（2）硫氧还蛋白基因簇；（3）复制区域；（4）与表皮葡萄球菌ATCC 12228的pSE - 12228 - 05高度同源的一个区域。nukacin ISK - 1生物合成和免疫基因簇中的基因组织与其他lacticin - 481型基因簇不同。复制区域编码的复制蛋白的特征与其他葡萄球菌θ复制质粒有所不同。pPI - 2包含一个抗消毒剂基因qacC，其完整DNA序列与其他qacC质粒如pSK108的序列显示出显著相似性，这表明pPI - 2属于qacC质粒组。
