Turni C, Blackall P J
Department of Primary Industries and Fisheries Queensland, Animal Research Institute, Locked Mail Bag No 4, Moorooka, Qld 4105, Australia.
Vet Microbiol. 2005 Mar 20;106(1-2):145-51. doi: 10.1016/j.vetmic.2004.12.019.
The aim of this study was to compare the use of indirect haemagglutination (IHA) and gel diffusion (GD) tests for serotyping Haemophilus parasuis by the Kielstein-Rapp-Gabrielson (KRG) scheme. All 15 serovar reference strains, 72 Australian field isolates, nine Chinese field isolates, and seven isolates from seven experimentally infected pigs were evaluated with both tests. With the IHA test, 14 of the 15 reference strains were correctly serotyped-with serovar 10 failing to give a titre with serovar 10 antiserum. In the GD test, 13 reference strains were correctly serotyped-with antigen from serovars 7 and 8 failing to react with any antiserum. The IHA methodology serotyped a total of 45 of 81 field isolates while the GD methodology serotyped a total of 48 isolates. For 29 isolates, the GD and IHA methods gave discordant results. It was concluded that the IHA is a good additional test for the serotyping of H. parasuis by the KRG scheme if the GD methodology fails to provide a result or shows unusual cross-reactions.
本研究的目的是比较采用基尔施泰因 - 拉普 - 加布里埃尔森（KRG）分型方案，运用间接血凝试验（IHA）和凝胶扩散试验（GD）对副猪嗜血杆菌进行血清分型的效果。使用这两种试验对所有15株血清型参考菌株、72株澳大利亚野外分离株、9株中国野外分离株以及7株来自7头实验感染猪的分离株进行了评估。在IHA试验中，15株参考菌株中有14株被正确血清分型，血清型10菌株与血清型10抗血清未出现滴度反应。在GD试验中，13株参考菌株被正确血清分型，血清型7和8的抗原与任何抗血清均未发生反应。IHA方法对81株野外分离株中的45株进行了血清分型，而GD方法对48株进行了血清分型。对于29株分离株，GD和IHA方法得出了不一致的结果。得出的结论是，如果GD方法未能得出结果或显示异常交叉反应，IHA是通过KRG方案对副猪嗜血杆菌进行血清分型的一种很好的补充试验。
