轮转运动改变大鼠中缝核中5-羟色胺（5-HT）转运体、5-HT1A、5-HT1B及α1b-肾上腺素能受体的信使核糖核酸。

Wheel running alters serotonin (5-HT) transporter, 5-HT1A, 5-HT1B, and alpha 1b-adrenergic receptor mRNA in the rat raphe nuclei.

作者信息

Greenwood Benjamin N, Foley Teresa E, Day Heidi E W, Burhans Daniel, Brooks Leah, Campeau Serge, Fleshner Monika

机构信息

Department of Integrative Physiology, University of Colorado, Boulder, Colorado 80309-0354, USA.

出版信息

Biol Psychiatry. 2005 Mar 1;57(5):559-68. doi: 10.1016/j.biopsych.2004.11.025.

DOI:10.1016/j.biopsych.2004.11.025

PMID:15737672

Abstract

BACKGROUND

Altered serotonergic (5-HT) neurotransmission is implicated in the antidepressant and anxiolytic properties of physical activity. In the current study, we investigated whether physical activity alters factors involved in the regulation of central 5-HT neural activity.

METHODS

In situ hybridization was used to quantify levels of 5-HT transporter (5-HTT), 5-HT(1A), 5-HT(1B), and alpha(1b)-adrenergic receptor (alpha(1b) ADR) messenger ribonucleic acids (mRNAs) in the dorsal (DRN) and median raphe (MR) nuclei of male Fischer rats after either sedentary housing or 3 days, 3 weeks, or 6 weeks of wheel running.

RESULTS

Wheel running produced a rapid and lasting reduction of 5-HT(1B) mRNA in the ventral DRN. Three weeks of wheel running decreased 5-HTT mRNA in the DRN and MR and increased alpha(1b) ADR mRNA in the DRN. After 6 weeks of wheel running, 5-HTT mRNA remained reduced, but alpha(1b) ADR mRNA returned to sedentary levels. Serotonin(1A) mRNA was increased in the MR and certain DRN subregions after 6 weeks only.

CONCLUSIONS

Data suggest that the central 5-HT system is sensitive to wheel running in a time-dependent manner. The observed changes in mRNA regulation in a subset of raphe nuclei might contribute to the stress resistance produced by wheel running and the antidepressant and anxiolytic effects of physical activity.

摘要

背景

血清素能（5-羟色胺，5-HT）神经传递的改变与体育活动的抗抑郁和抗焦虑特性有关。在本研究中，我们调查了体育活动是否会改变参与调节中枢5-HT神经活动的因素。

方法

采用原位杂交技术，对雄性Fischer大鼠在固定饲养或进行3天、3周或6周轮转运动后，其背侧中缝核（DRN）和中缝正中核（MR）中5-HT转运体（5-HTT）、5-HT（1A）、5-HT（1B）和α（1b）-肾上腺素能受体（α（1b）ADR）信使核糖核酸（mRNA）的水平进行定量分析。

结果

轮转运动使腹侧DRN中5-HT（1B）mRNA迅速且持续减少。3周的轮转运动使DRN和MR中的5-HTT mRNA减少，DRN中的α（1b）ADR mRNA增加。6周的轮转运动后，5-HTT mRNA仍保持减少状态，但α（1b）ADR mRNA恢复到固定饲养水平。仅在6周后，MR和某些DRN亚区域中的5-羟色胺（1A）mRNA增加。