Polo Sophie E, Almouzni Geneviève
Laboratory of Nuclear Dynamics and Genome Plasticity, UMR 218 CNRS/Institut Curie, 26 rue d'Ulm, 75248 Paris cedex 5, France.
Cancer Lett. 2005 Mar 18;220(1):1-9. doi: 10.1016/j.canlet.2004.08.024.
The structural organization of DNA into chromatin is of key importance to regulate genome function and stability. Maintenance of such an organization is thus crucial to preserve cellular identity. At each cell cycle, during S phase, this is achieved by duplication of chromatin structure in tight coordination with DNA replication. Such a coordinate process requires histone synthesis and their deposition onto DNA by chromatin assembly factors to be efficiently coupled to DNA synthesis. In this review, we highlight the intimate relationship between these chromatin-related events and DNA replication and we show how it is possible to take advantage of this coupling in order to identify cells with high replicative potential such as tumor cells. On the basis of recent data, we discuss the potential use of chromatin-associated factors as new proliferation markers of interest for cancer diagnosis and prognosis.
DNA构建成染色质的结构组织对于调节基因组功能和稳定性至关重要。因此，维持这种组织结构对于保持细胞特性至关重要。在每个细胞周期的S期，这是通过染色质结构的复制与DNA复制紧密协调来实现的。这样一个协调过程需要组蛋白合成以及染色质组装因子将它们沉积到DNA上，并有效地与DNA合成相耦合。在这篇综述中，我们强调了这些与染色质相关的事件和DNA复制之间的密切关系，并展示了如何利用这种耦合来识别具有高复制潜力的细胞，如肿瘤细胞。基于最近的数据，我们讨论了染色质相关因子作为癌症诊断和预后新的增殖标志物的潜在用途。
