Nakamura Hiroyuki, Mukai Mutsuko, Komatsu Keiko, Tanaka-Okamoto Miki, Itoh Yu, Ishizaki Hiroyoshi, Tatsuta Masaharu, Inoue Masahiro, Miyoshi Jun
Department of Tumor Biochemistry, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3 Nakamichi, Higashinari-ku, Osaka 537-8511, Japan.
Cancer Lett. 2005 Mar 18;220(1):95-9. doi: 10.1016/j.canlet.2004.07.023.
We have previously shown that transforming growth factor-beta1 (TGF-beta1) markedly stimulates the invasive capacity of rat ascites hepatoma AH130 W1 cells in vitro and in vivo. A differential hybridization procedure was used to isolate genes that were specifically up-regulated in TGF-beta1 treated W1 cells. Among ten independent cDNA clones, we focused on LMO7 and a variant isoform, LMO7S, that was generated by alternative splicing. LMO7 had PDZ and LIM domains, while LMO7S had only PDZ domain. TGF-beta1 up-regulated expression levels of LMO7 and LMO7S. LMO7 expression was up-regulated in the highly metastatic clone MM1.
我们之前已经表明，转化生长因子-β1（TGF-β1）在体外和体内均能显著刺激大鼠腹水肝癌AH130 W1细胞的侵袭能力。采用差异杂交方法分离在TGF-β1处理的W1细胞中特异性上调的基因。在十个独立的cDNA克隆中，我们重点研究了LMO7和一个可变剪接产生的异构体LMO7S。LMO7具有PDZ和LIM结构域，而LMO7S仅具有PDZ结构域。TGF-β1上调了LMO7和LMO7S的表达水平。在高转移性克隆MM1中，LMO7的表达上调。
