Zheng P, Xie X, Liu P
Department of Chemistry, Yunnan Normal University, Kunming, 650092.
Se Pu. 1997 Nov;15(6):539-41.
In this paper a high performance liquid chromatographic method for the separation and determination of metsulfuron, bensulfuron and acetochlor in the composite herbicide "Nongjiayi" is described. The concentration of methanol in the mobile phase and the temperature of the column affected the k' factor and the separation of the three active ingredients. Impurities in the "Nongjiayi" did not interfere with the determination. Chromatographic conditions were Shim-Pack CLC-C8 (150 mm x 6 mm i.d., 5 microm), mobile phase of methanol-water (60:40, V/V) and UV detector at 240 nm. Quantification was carried out with the three external standards. The average recovery was 98.81% with RSD=0.80% for metsulfuron, 99.10% with RSD=0.78% for bensulfuron and 99.72% with RSD=0.69% for acetochlor. The linear range of this method was 0.10-0.40 microg (r=0.9994) for metsulfuron, 0.40-1.60 microg (r=0.9995) for bensulfuron and 10-40 microg (r=0.9987) for acetochlor. The method is accurate, rapid and reproducible and can be applied to quality control for the "Nongjiayi".
本文描述了一种用于分离和测定复合除草剂“农佳一”中甲磺隆、苄嘧磺隆和乙草胺的高效液相色谱法。流动相中甲醇的浓度和柱温影响k'因子以及三种活性成分的分离。“农佳一”中的杂质不干扰测定。色谱条件为Shim-Pack CLC-C8（150 mm×6 mm内径，5微米），甲醇-水（60:40，V/V）流动相，240 nm紫外检测器。采用三种外标进行定量。甲磺隆的平均回收率为98.81%，相对标准偏差为0.80%；苄嘧磺隆的平均回收率为99.10%，相对标准偏差为0.78%；乙草胺的平均回收率为99.72%，相对标准偏差为0.69%。该方法中甲磺隆的线性范围为0.10 - 0.40微克（r = 0.9994），苄嘧磺隆为0.40 - 1.60微克（r = 0.9995），乙草胺为10 - 40微克（r = 0.9987）。该方法准确、快速且可重复，可用于“农佳一”的质量控制。
