Li G, Fan C, Xing C
Department of Chemical Engineering, Shandong Institute of Light Industry, Jinan, 250100.
Se Pu. 1997 Jan;15(1):65-6.
In this paper, separation of water soluble vitamins C (VC), B1 (VB1) and B6 (VB6) has been studied preliminarily by capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MECC) with a home-made amperometric electrochemical detector at the working electrode [carbon fiber (5 microm o.d.)] potential of 510-540 mV (vs. SCE). It has been shown that each of the three vitamins has more than one peak in NH3-NH4Cl (pH 9.0) which were mainly caused by the decomposition products in weak base medium and by heat except those from impurities. The electrochemical detector has better performance for the three vitamins from CZE and MECC. They can be separated either by CZE or by MECC. The separation by CZE is better than that by MECC with sodium dodecyl sulfate (SDS). SDS and buffer acidity can influence the retention time, peak sequence and height. The separation efficiency of CZE in 0.01 mol/L NH3-NH4Cl was 468 800 theoretical plates for VC.
本文采用自制的安培电化学检测器，在工作电极[外径5微米的碳纤维]电位为510 - 540 mV（相对于饱和甘汞电极）的条件下，利用毛细管区带电泳（CZE）和胶束电动毛细管色谱法（MECC）对水溶性维生素C（VC）、维生素B1（VB1）和维生素B6（VB6）的分离进行了初步研究。结果表明，在NH3 - NH4Cl（pH 9.0）中，这三种维生素各自均出现多个峰，除杂质峰外，主要是由弱碱介质中的分解产物及热导致的。电化学检测器对CZE和MECC分离出的这三种维生素具有较好的检测性能。它们既可以通过CZE分离，也可以通过MECC分离。CZE的分离效果优于使用十二烷基硫酸钠（SDS）的MECC。SDS和缓冲液酸度会影响保留时间、峰序和峰高。在0.01 mol/L NH3 - NH4Cl中，CZE对VC的分离效率为468 800理论塔板数。
