[Preliminary study on separation of three vitamins by capillary zone electrophoresis and micellar electrokinetic chromatography with amperometric electrochemical detection].

In this paper, separation of water soluble vitamins C (VC), B1 (VB1) and B6 (VB6) has been studied preliminarily by capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MECC) with a home-made amperometric electrochemical detector at the working electrode [carbon fiber (5 microm o.d.)] potential of 510-540 mV (vs. SCE). It has been shown that each of the three vitamins has more than one peak in NH3-NH4Cl (pH 9.0) which were mainly caused by the decomposition products in weak base medium and by heat except those from impurities. The electrochemical detector has better performance for the three vitamins from CZE and MECC. They can be separated either by CZE or by MECC. The separation by CZE is better than that by MECC with sodium dodecyl sulfate (SDS). SDS and buffer acidity can influence the retention time, peak sequence and height. The separation efficiency of CZE in 0.01 mol/L NH3-NH4Cl was 468 800 theoretical plates for VC.