Coolen S A, Huf F A, Reijenga J C
Department of Chemistry, University of Technology, Eindhoven, The Netherlands.
J Chromatogr B Biomed Sci Appl. 1998 Oct 9;717(1-2):119-24. doi: 10.1016/s0378-4347(98)00289-8.
Hydroxylated radical products of salicylic acid are often used as a relative measurement in free radical research. Several analytical methods exist to determine the amount of 2,3-dihydroxybenzoic acid and 2,5-dihydroxybenzoic acid. In this study we use capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MECC) in order to determine these free radical products. The CZE experiment was optimized with a CZE simulation program HPCESIM in order to achieve an optimal pH. Calibration curves were recorded in the range 10(-6)-10(-4) M and the detection limit was determined. For both CZE and MECC it was 2x10(-7) M. Both methods resulted in a reproducible analysis of salicylate and its hydroxylated free radical products in 6 min.
水杨酸的羟基化自由基产物常用于自由基研究中的相对测量。存在多种分析方法来测定2,3 - 二羟基苯甲酸和2,5 - 二羟基苯甲酸的含量。在本研究中，我们使用毛细管区带电泳（CZE）和胶束电动毛细管色谱法（MECC）来测定这些自由基产物。利用CZE模拟程序HPCESIM对CZE实验进行了优化，以实现最佳pH值。记录了10(-6)-10(-4) M范围内的校准曲线并确定了检测限。对于CZE和MECC，检测限均为2×10(-7) M。两种方法都能在6分钟内对水杨酸盐及其羟基化自由基产物进行可重复分析。
