Zhang Q, Wu C, Cao J, Xue H
Beijing Institute of Radiation Medicine, Beijing, 100850.
Se Pu. 1997 Jan;15(1):79-80.
7-Ketocholesterol (7-KC), which is a major oxidation product of cholesterol and is selective cytotoxic to tumour cells, was isolated from human fetal liver. A fast, sensitive method of high performance liquid chromatography for the quantitation of 7-KC has been developed and applied to the determination of 7-KC in human fetal liver cell suspension. In this procedure a mixture of 2 : 1 chloroform-methanol (V/V) was used as extraction solvent. The extract was washed with distilled water and then evaporated to dryness under N2. An adsorption liquid chromatographic system used included mu-Porasil SiO2 column, hexane : iso-propanol (91 : 9) mobile phase and UV detector at 233nm. It was found that the level of 7-KC in human fetal liver cell supernatant was higher than in liver lysate.
7-酮胆固醇（7-KC）是胆固醇的主要氧化产物，对肿瘤细胞具有选择性细胞毒性，它是从人胎儿肝脏中分离出来的。已开发出一种快速、灵敏的高效液相色谱法用于定量7-KC，并将其应用于人胎儿肝细胞悬液中7-KC的测定。在此方法中，使用2:1的氯仿-甲醇（V/V）混合物作为萃取溶剂。提取物用蒸馏水洗涤，然后在氮气下蒸发至干。所使用的吸附液相色谱系统包括μ- Porasil SiO₂柱、己烷:异丙醇（91:9）流动相和233nm的紫外检测器。结果发现，人胎儿肝细胞上清液中7-KC的水平高于肝脏裂解液中的水平。
