Zhang Q L, Wu Z Z, Cao J R, Xue H H, Pei X T, Wang L S, Xu L, Du Z H, Feng R
Beijing Institute of Radiation Medicine.
Sheng Li Xue Bao. 1997 Jun;49(3):327-32.
Our previous work showed the existence of low molecular weight tumor suppressors in human fetal tissues. In this paper, two tumor cell suppressors were isolated and purified from methanol extract of human fetal liver by C18 reversed-phase medium pressure chromatography, gel filtration on Sephadex LH-20, and high-performance liquid chromatography, directed by suppression of growth of HL-60 cells. The structures of the suppressors were identified to be 7-ketocholesterol and 7-beta-hydroxycholesterol. Under the condition of in vitro agar plate culture, 7-ketocholesterol and 7-beta-hydroxycholesterol showed preferentially inhibitory effects on the growth of both human and murine leukemic cell lines including human HL-60 and murine S-180 cells, but less effective on the growth of normal human and murine bone marrow granulocyte-macrophage progenitors (CFU-GM).
我们之前的研究表明，人类胎儿组织中存在低分子量肿瘤抑制因子。本文通过C18反相中压色谱、Sephadex LH - 20凝胶过滤和高效液相色谱，从人胎儿肝脏的甲醇提取物中分离并纯化出两种肿瘤细胞抑制因子，其分离过程以对HL - 60细胞生长的抑制作用为导向。经鉴定，这些抑制因子的结构为7-酮胆固醇和7-β-羟基胆固醇。在体外琼脂平板培养条件下，7-酮胆固醇和7-β-羟基胆固醇对包括人HL - 60和鼠S - 180细胞在内的人和鼠白血病细胞系的生长均表现出优先抑制作用，但对正常人和鼠骨髓粒-巨噬细胞祖细胞（CFU - GM）的生长抑制作用较弱。
